Endothelin-1 Stimulates Human Monocytes in Vitro to Release TNF-alpha , IL-1beta and IL-6

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 1993 Jan 1

PMID 18475557

Citations 12

Authors

E Helset

T Sildnes

R Seljelid

Z S Konopski

Affiliations

Soon will be listed here.

Abstract

Increased plasma- and tissue levels of endothelin-1 (ET-1) during inflammatory diseases, have suggested a role of ET-1 in the pathophysiology of inflammatory reactions. The authors have studied the effect of ET-1 on cytokine release from monocytes and monocyte-derived macrophages. ET-1 increased secretion of TNF-alpha, IL-1beta and IL-6 in a dose- and time-dependent manner. Optimal ET-1 concentration ranged from 0.01 to 1 nM. The maximal response was a 200 to 400% increase in cytokine release. A time-course study revealed that the pattern of cytokines induced by ET-1 was different in monocytes and macrophages, although an early increase in TNF-alpha was observed in both monocyte and macrophage supernatants. In conclusion, ET-1 stimulates monocytes and macrophages to release cytokines thereby demonstrating a potential role for ET-1 in regulation of inflammatory responses.

Citing Articles

Predictive Value of Selected Plasma Biomarkers in the Assessment of the Occurrence and Severity of Coronary Artery Disease.

Wojciechowska M, Nizio M, Wrobel K, Momot K, Czarzasta K, Flis K Int J Mol Sci. 2025; 26(2).

PMID: 39859253 PMC: 11765375. DOI: 10.3390/ijms26020537.

Molecular Signature of Monocytes Shaped by the 1790-Generalized Modules for Membrane Antigens Vaccine.

Tondi S, Siena E, Essaghir A, Bozzetti B, Bechtold V, Scaillet A Int J Mol Sci. 2024; 25(2).

PMID: 38256189 PMC: 10816432. DOI: 10.3390/ijms25021116.

Serum Biomarkers in Connective Tissue Disease-Associated Pulmonary Arterial Hypertension.

Moccaldi B, De Michieli L, Binda M, Famoso G, Depascale R, Perazzolo Marra M Int J Mol Sci. 2023; 24(4).

PMID: 36835590 PMC: 9967966. DOI: 10.3390/ijms24044178.

NLRP3 activation contributes to endothelin-1-induced erectile dysfunction.

Sobrano Fais R, Menezes da Costa R, Carvalho Mendes A, Mestriner F, Comerma-Steffensen S, Tostes R J Cell Mol Med. 2022; 27(1):1-14.

PMID: 36515571 PMC: 9806301. DOI: 10.1111/jcmm.17463.

Endothelin B Receptor Immunodynamics in Pulmonary Arterial Hypertension.

Tabeling C, Gonzalez Calera C, Lienau J, Hoppner J, Tschernig T, Kershaw O Front Immunol. 2022; 13:895501.

PMID: 35757687 PMC: 9221837. DOI: 10.3389/fimmu.2022.895501.

References

Schindler R, Mancilla J, Endres S, Ghorbani R, Clark S, Dinarello C . Correlations and interactions in the production of interleukin-6 (IL-6), IL-1, and tumor necrosis factor (TNF) in human blood mononuclear cells: IL-6 suppresses IL-1 and TNF. Blood. 1990; 75(1):40-7. View

Dinarello C, Cannon J, Wolff S, Bernheim H, Beutler B, Cerami A . Tumor necrosis factor (cachectin) is an endogenous pyrogen and induces production of interleukin 1. J Exp Med. 1986; 163(6):1433-50. PMC: 2188124. DOI: 10.1084/jem.163.6.1433. View

Sugiura M, Inagami T, Kon V . Endotoxin stimulates endothelin-release in vivo and in vitro as determined by radioimmunoassay. Biochem Biophys Res Commun. 1989; 161(3):1220-7. DOI: 10.1016/0006-291x(89)91372-7. View

Johnson J, Meyrick B, Jesmok G, Brigham K . Human recombinant tumor necrosis factor alpha infusion mimics endotoxemia in awake sheep. J Appl Physiol (1985). 1989; 66(3):1448-54. DOI: 10.1152/jappl.1989.66.3.1448. View

Fong Y, Tracey K, Moldawer L, Hesse D, Manogue K, KENNEY J . Antibodies to cachectin/tumor necrosis factor reduce interleukin 1 beta and interleukin 6 appearance during lethal bacteremia. J Exp Med. 1989; 170(5):1627-33. PMC: 2189514. DOI: 10.1084/jem.170.5.1627. View

Yoshizumi M, Kurihara H, Sugiyama T, Takaku F, Yanagisawa M, Masaki T . Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells. Biochem Biophys Res Commun. 1989; 161(2):859-64. DOI: 10.1016/0006-291x(89)92679-x. View

Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y . A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature. 1988; 332(6163):411-5. DOI: 10.1038/332411a0. View

Gearing A, Bird C, Bristow A, Poole S, Thorpe R . A simple sensitive bioassay for interleukin-1 which is unresponsive to 10(3) U/ml of interleukin-2. J Immunol Methods. 1987; 99(1):7-11. DOI: 10.1016/0022-1759(87)90025-1. View

Pittet J, Morel D, Hemsen A, Gunning K, Lacroix J, Suter P . Elevated plasma endothelin-1 concentrations are associated with the severity of illness in patients with sepsis. Ann Surg. 1991; 213(3):261-4. PMC: 1358338. DOI: 10.1097/00000658-199103000-00014. View

10.

Helset E, Kjaeve J, Hauge A . Endothelin-1-induced increases in microvascular permeability in isolated, perfused rat lungs requires leukocytes and plasma. Circ Shock. 1993; 39(1):15-20. View

11.

Espevik T, Nissen-Meyer J . A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. J Immunol Methods. 1986; 95(1):99-105. DOI: 10.1016/0022-1759(86)90322-4. View

12.

Murch S, Braegger C, Sessa W, MacDonald T . High endothelin-1 immunoreactivity in Crohn's disease and ulcerative colitis. Lancet. 1992; 339(8790):381-5. DOI: 10.1016/0140-6736(92)90077-g. View

13.

Luscher T, Boulanger C, Dohi Y, Yang Z . Endothelium-derived contracting factors. Hypertension. 1992; 19(2):117-30. DOI: 10.1161/01.hyp.19.2.117. View

14.

Ehrenreich H, Burd P, Rottem M, Hultner L, Hylton J, Garfield M . Endothelins belong to the assortment of mast cell-derived and mast cell-bound cytokines. New Biol. 1992; 4(2):147-56. View

15.

Ehrenreich H, Anderson R, Fox C, Rieckmann P, Hoffman G, Travis W . Endothelins, peptides with potent vasoactive properties, are produced by human macrophages. J Exp Med. 1990; 172(6):1741-8. PMC: 2188743. DOI: 10.1084/jem.172.6.1741. View

16.

Miyasaka N, Hirata Y, Ando K, Sato K, Morita H, Shichiri M . Increased production of endothelin-1 in patients with inflammatory arthritides. Arthritis Rheum. 1992; 35(4):397-400. DOI: 10.1002/art.1780350406. View

17.

Billiau A, Vandekerckhove F . Cytokines and their interactions with other inflammatory mediators in the pathogenesis of sepsis and septic shock. Eur J Clin Invest. 1991; 21(6):559-73. DOI: 10.1111/j.1365-2362.1991.tb01410.x. View

18.

Hieda H, Gomez-Sanchez C . Hypoxia increases endothelin release in bovine endothelial cells in culture, but epinephrine, norepinephrine, serotonin, histamine and angiotensin II do not. Life Sci. 1990; 47(3):247-51. DOI: 10.1016/0024-3205(90)90327-n. View

19.

Waage A, Aasen A . Different role of cytokine mediators in septic shock related to meningococcal disease and surgery/polytrauma. Immunol Rev. 1992; 127:221-30. DOI: 10.1111/j.1600-065x.1992.tb01416.x. View

20.

Boyum A . Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968; 97:77-89. View