Systemic Oxidative Stress in Children with Cystic Fibrosis with Bacterial Infection Including Pseudomonas Aeruginosa

Overview

Journal Clin Respir J

Specialty Pulmonary Medicine

Date 2022 Jun 26

PMID 35753706

Authors

Gabriela Datsch Bennemann

Emilia Addison Machado Moreira

Leticia Cristina Radin Pereira

Maiara Brusco de Freitas

Diane de Oliveira

Julia Carvalho Ventura

Eduardo Benedetti Parisotto

Yara Maria Franco Moreno

Erasmo Benicio Santos Moraes Trindade

Eliana Barbosa

Norberto Ludwig Neto

Danilo Wilhelm Filho

Affiliations

Soon will be listed here.

Abstract

Introduction: Oxidative stress (OS) occurs in cystic fibrosis (CF).

Objective: The objective of this work is to evaluate the influence of bacterial infection on biomarkers of OS (catalase [CAT], glutathione peroxidade [GPx], reduced glutathione [GSH]), markers of oxidative damage (protein carbonyls [PC], thiobarbituric acid reactive substances [TBARS]), together with the nutritional status and lung function in children with CF.

Methods: Cross-sectional study including CF group (CFG, n = 55) and control group (CG, n = 31), median age: 3.89 and 4.62 years, respectively. CFG was distributed into CFG negative bacteriology (CFGB-, n = 27) or CFG positive bacteriology (CFGB+, n = 28), and CFG negative Pseudomonas aeruginosa (CFGPa-, n = 36) or CFG positive Pseudomonas aeruginosa (CFGPa+, n = 19).

Results: Compared with CG, CFG (P = .034) and CFGB+ (P = .042) had lower body mass index-for-age z-score; forced expiratory volume in the first second was lower in CFGB+ and CFGPa+ (both P < .001). After adjusting for confounders and compared with CG: CFG showed higher TBARS (P ≤ .001) and PC (P = .048), and lower CAT (P = .004) and GPx (P = .003); the increase in PC levels was observed in CFGB+ (P = .011) and CFGPa+ (P = .001) but not in CFGB- (P = .510) and CFGPa- (P = .460).

Conclusions: These results indicate a systemic OS in children with CF. The presence of bacterial infection particularly Pseudomonas aeruginosa seems to be determinant to exacerbate the oxidative damage to proteins, in which PC may be a useful biomarker of OS in CF.

Citing Articles

Systemic oxidative stress in children with cystic fibrosis with bacterial infection including Pseudomonas Aeruginosa.

Bennemann G, Moreira E, Pereira L, de Freitas M, de Oliveira D, Ventura J Clin Respir J. 2022; 16(6):475-483.

PMID: 35753706 PMC: 9366585. DOI: 10.1111/crj.13513.

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