Stress Buffering and Longevity Effects of Amber Extract on ()

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Jun 24

PMID 35744983

Authors

Sandra Somuah-Asante

Kazuichi Sakamoto

Affiliations

Soon will be listed here.

Abstract

Amber is a fossilized tree resin historically used in wound healing and stress relief. Unfortunately, there is no concrete scientific evidence supporting such efficacy. Here, the stress buffering and longevity effect of Amber extract (AE) in () was investigated. Survival assays, health span assays, Enzyme-Linked Immunosorbent Assay (ELISA), Stress biomarker detection assays, Green Fluorescence Proteins (GFP), Real Time quantitative PCR (RT-qPCR) and mutants were employed to investigate the stress buffering and longevity effect of AE. In the study, it was observed that AE supplementation improved health span and survival in both normal and stressed worms. Additionally, AE positively regulated stress hormones (cortisol, oxytocin, and dopamine) and decreased fat and reactive oxygen species (ROS) accumulation. Through the Insulin/IGF-1 signaling (IIS) pathway, AE enhanced the nuclear localization of DAF-16 and the expression of heat shock proteins and antioxidant genes in GFP-tagged worms and at messenger RNA levels. Finally, AE failed to increase the survival of , , and loss-of-function mutants, confirming the involvement of the IIS pathway. Evidently, AE supplementation relieves stress and enhances longevity. Thus, amber may be a potent nutraceutical for stress relief.

Citing Articles

L-Theanine Prolongs the Lifespan by Activating Multiple Molecular Pathways in Ultraviolet C-Exposed .

Chen L, Chen G, Gai T, Zhou X, Zhu J, Wang R Molecules. 2024; 29(11).

PMID: 38893565 PMC: 11173996. DOI: 10.3390/molecules29112691.

Ovalbumin promotes innate immune response of Caenorhabditis elegans through DAF-16 and SKN-1 pathways in insulin/IGF-1 signaling.

Pei H, Lin Z, Yao K, Luo Y, Tong P, Chen H J Physiol Biochem. 2024; 80(3):541-559.

PMID: 38632209 DOI: 10.1007/s13105-024-01021-2.

References

Murphy C, McCarroll S, Bargmann C, Fraser A, Kamath R, Ahringer J . Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature. 2003; 424(6946):277-83. DOI: 10.1038/nature01789. View

Landis G, Tower J . Superoxide dismutase evolution and life span regulation. Mech Ageing Dev. 2005; 126(3):365-79. DOI: 10.1016/j.mad.2004.08.012. View

Ozbay F, Fitterling H, Charney D, Southwick S . Social support and resilience to stress across the life span: a neurobiologic framework. Curr Psychiatry Rep. 2008; 10(4):304-10. DOI: 10.1007/s11920-008-0049-7. View

Dehghan E, Zhang Y, Saremi B, Yadavali S, Hakimi A, Dehghani M . Hydralazine induces stress resistance and extends C. elegans lifespan by activating the NRF2/SKN-1 signalling pathway. Nat Commun. 2017; 8(1):2223. PMC: 5738364. DOI: 10.1038/s41467-017-02394-3. View

Sugawara T, Sakamoto K . Quercetin enhances motility in aged and heat-stressed Caenorhabditis elegans nematodes by modulating both HSF-1 activity, and insulin-like and p38-MAPK signalling. PLoS One. 2020; 15(9):e0238528. PMC: 7470330. DOI: 10.1371/journal.pone.0238528. View

Tian Y, Zhou S, Takeda R, Okazaki K, Sekita M, Sakamoto K . Anti-inflammatory activities of amber extract in lipopolysaccharide-induced RAW 264.7 macrophages. Biomed Pharmacother. 2021; 141:111854. DOI: 10.1016/j.biopha.2021.111854. View

Kim S, Kim B, Park S, Park S . Phosphatidylcholine Extends Lifespan via DAF-16 and Reduces Amyloid-Beta-Induced Toxicity in . Oxid Med Cell Longev. 2019; 2019:2860642. PMC: 6657616. DOI: 10.1155/2019/2860642. View

McQuaid R, McInnis O, Paric A, Al-Yawer F, Matheson K, Anisman H . Relations between plasma oxytocin and cortisol: The stress buffering role of social support. Neurobiol Stress. 2016; 3:52-60. PMC: 5146198. DOI: 10.1016/j.ynstr.2016.01.001. View

Sogo E, Zhou S, Haeiwa H, Takeda R, Okazaki K, Sekita M . Amber Extract Reduces Lipid Content in Mature 3T3-L1 Adipocytes by Activating the Lipolysis Pathway. Molecules. 2021; 26(15). PMC: 8348738. DOI: 10.3390/molecules26154630. View

10.

Altintas O, Park S, Lee S . The role of insulin/IGF-1 signaling in the longevity of model invertebrates, C. elegans and D. melanogaster. BMB Rep. 2015; 49(2):81-92. PMC: 4915121. DOI: 10.5483/bmbrep.2016.49.2.261. View

11.

Forman H, Augusto O, Brigelius-Flohe R, Dennery P, Kalyanaraman B, Ischiropoulos H . Even free radicals should follow some rules: a guide to free radical research terminology and methodology. Free Radic Biol Med. 2014; 78:233-5. DOI: 10.1016/j.freeradbiomed.2014.10.504. View

12.

Rangsinth P, Prasansuklab A, Duangjan C, Gu X, Meemon K, Wink M . Leaf extract of Caesalpinia mimosoides enhances oxidative stress resistance and prolongs lifespan in Caenorhabditis elegans. BMC Complement Altern Med. 2019; 19(1):164. PMC: 6615182. DOI: 10.1186/s12906-019-2578-5. View

13.

Lin C, Chen Y, Lin Y, Wang X, Hu L, Cao Y . Antistress and anti-aging activities of Caenorhabditis elegans were enhanced by Momordica saponin extract. Eur J Nutr. 2020; 60(4):1819-1832. DOI: 10.1007/s00394-020-02338-6. View

14.

ORourke E, Soukas A, Carr C, Ruvkun G . C. elegans major fats are stored in vesicles distinct from lysosome-related organelles. Cell Metab. 2009; 10(5):430-5. PMC: 2921818. DOI: 10.1016/j.cmet.2009.10.002. View

15.

McEwen B . Central effects of stress hormones in health and disease: Understanding the protective and damaging effects of stress and stress mediators. Eur J Pharmacol. 2008; 583(2-3):174-85. PMC: 2474765. DOI: 10.1016/j.ejphar.2007.11.071. View

16.

Escorcia W, Ruter D, Nhan J, Curran S . Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining. J Vis Exp. 2018; (133). PMC: 5931440. DOI: 10.3791/57352. View

17.

Sugawara T, Sakamoto K . Killed Bifidobacterium longum enhanced stress tolerance and prolonged life span of Caenorhabditis elegans via DAF-16. Br J Nutr. 2018; 120(8):872-880. DOI: 10.1017/S0007114518001563. View

18.

Lithgow G, Walker G . Stress resistance as a determinate of C. elegans lifespan. Mech Ageing Dev. 2002; 123(7):765-71. DOI: 10.1016/s0047-6374(01)00422-5. View

19.

Ranabir S, Reetu K . Stress and hormones. Indian J Endocrinol Metab. 2011; 15(1):18-22. PMC: 3079864. DOI: 10.4103/2230-8210.77573. View

20.

Baumeister R, Schaffitzel E, Hertweck M . Endocrine signaling in Caenorhabditis elegans controls stress response and longevity. J Endocrinol. 2006; 190(2):191-202. DOI: 10.1677/joe.1.06856. View