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From Methylome to Integrative Analysis of Tissue Specificity

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Specialty Molecular Biology
Date 2022 Jun 22
PMID 35732948
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Abstract

DNA methylation is the most studied epigenetic mark in both plants and animals. The gold standard for assaying genome-wide DNA methylation at single-base resolution is whole-genome bisulfite sequencing (WGBS). Here, we describe an improved procedure for WGBS and original bioinformatic workflows applied to unravel tissue-specific variations of the methylome in relation to gene expression and accumulation of secondary metabolites in the medicinal plant Catharanthus roseus.

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Beadchip technology to detect DNA methylation in mouse faithfully recapitulates whole-genome bisulfite sequencing.

Martin E, Grimm S, Xu Z, Taylor J, Wade P Epigenomics. 2023; 15(3):115-129.

PMID: 37020391 PMC: 10131490. DOI: 10.2217/epi-2023-0034.

References
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