Ecology of Methanonatronarchaeia

Overview

Journal Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2022 Jun 21

PMID 35726892

Authors

Dimitry Y Sorokin

Alexander Y Merkel

Ben Abbas

Affiliations

Soon will be listed here.

Abstract

Methanonatronarchaeia represents a deep-branching phylogenetic lineage of extremely halo(alkali)philic and moderately thermophilic methyl-reducing methanogens belonging to the phylum Halobacteriota. It includes two genera, the alkaliphilic Methanonatronarchaeum and the neutrophilic Ca. Methanohalarchaeum. The former is represented by multiple closely related pure culture isolates from hypersaline soda lakes, while the knowledge about the latter is limited to a few mixed cultures with anaerobic haloarchaea. To get more insight into the distribution and ecophysiology of this enigmatic group of extremophilic methanogens, potential activity tests and enrichment cultivation with different substrates and at different conditions were performed with anaerobic sediment slurries from various hypersaline lakes in Russia. Methanonatronarchaeum proliferated exclusively in hypersaline soda lake samples mostly at elevated temperature, while at mesophilic conditions it coexisted with the extremely salt-tolerant methylotroph Methanosalsum natronophilum. Methanonatronarchaeum was also able to serve as a methylotrophic or hydrogenotrophic partner in several thermophilic enrichment cultures with fermentative bacteria. Ca. Methanohalarchaeum did not proliferate at mesophilic conditions and at thermophilic conditions it competed with extremely halophilic and moderately thermophilic methylotroph Methanohalobium, which it outcompeted at a combination of elevated temperature and methyl-reducing conditions. Overall, the results demonstrated that Methanonatronarchaeia are specialized extremophiles specifically proliferating in conditions of elevated temperature coupled with extreme salinity and simultaneous availability of a wide range of C -methylated compounds and H /formate.

Citing Articles

Highly diverse-Low abundance methanogenic communities in hypersaline microbial mats of Guerrero Negro B.C.S., assessed through microcosm experiments.

Ramirez-Arenas P, Latisnere-Barragan H, Garcia-Maldonado J, Lopez-Cortes A PLoS One. 2024; 19(10):e0303004.

PMID: 39365803 PMC: 11451985. DOI: 10.1371/journal.pone.0303004.

Ecology of Methanonatronarchaeia.

Sorokin D, Merkel A, Abbas B Environ Microbiol. 2022; 24(11):5217-5229.

PMID: 35726892 PMC: 9796771. DOI: 10.1111/1462-2920.16108.

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