Differential Effects of Excess High-fructose Corn Syrup on the DNA Methylation of Hippocampal Neurotrophic Factor in Childhood and Adolescence

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2022 Jun 17

PMID 35714129

Authors

Itsuki Kageyama

Hiroya Yamada

Eiji Munetsuna

Mirai Yamazaki

Yoshitaka Ando

Genki Mizuno

Ryosuke Fujii

Yuki Nouchi

Takuya Wakasugi

Tomohide Sakakibara

Atsushi Teshigawara

Hiroaki Ishikawa

Yohei Shimono

Koji Suzuki

Shuji Hashimoto

Koji Ohashi

Affiliations

Soon will be listed here.

Abstract

Consumption of fructose-containing beverages such as high-fructose corn syrup (HFCS) is increasing, raising concerns about the negative effects of excessive fructose intake. A recent report indicated that excess HFCS intake impairs hippocampal function. In this study, we focused on neurotrophic factors (NFs) in the hippocampus from the viewpoint of epigenetics to clarify the adverse effects of fructose. We analyzed the effects of HFCS intake on hippocampal function in three age categories: childhood and adolescence (postnatal day (PD) 21-60), young adulthood (PD60-100), and late adulthood (PD100-140). For the experiments, male Sprague-Dawley rats were divided into three age categories, the control group was received distilled water and the HFCS group was received 20% HFCS solution for 40 days in each period. We analyzed mRNA and protein levels for qPCR and western blotting, respectively, of a hippocampal NF, brain-derived neurotrophic factor (Bdnf). HFCS consumption reduced hippocampal Bdnf mRNA and protein expressions in childhood and adolescence. Moreover, pyrosequencing assays revealed increased DNA methylation at the Bdnf promoter in childhood and adolescence. This Bdnf levels reduction may be due to hypermethylation of the promoter regions. It should be noted that this phenomenon was observed only in childhood and adolescence fructose consumption. Our results indicate that the sensitivity of the hippocampus to fructose may vary with age. This study provides insight into the adverse effects of excessive HFCS consumption on the hippocampus in children.

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