BTDAzo: A Photoswitchable TRPC5 Channel Activator

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2022 Jun 17

PMID 35713469

Authors

Markus Muller

Konstantin Niemeyer

Nicole Urban

Navin K Ojha

Frank Zufall

Trese Leinders-Zufall

Michael Schaefer

Oliver Thorn-Seshold

Affiliations

Soon will be listed here.

Abstract

Photoswitchable reagents can be powerful tools for high-precision biological control. TRPC5 is a Ca -permeable cation channel with distinct tissue-specific roles, from synaptic function to hormone regulation. Reagents giving spatiotemporally-resolved control over TRPC5 activity may be key to understanding and harnessing its biology. Here we develop the first photoswitchable TRPC5-modulator, BTDAzo, to address this goal. BTDAzo can photocontrol TRPC5 currents in cell culture, as well as controlling endogenous TRPC5-based neuronal Ca responses in mouse brain slices. BTDAzos are also the first reported azo-benzothiadiazines, an accessible and conveniently derivatised azoheteroarene with strong two-colour photoswitching. BTDAzo's ability to control TRPC5 across relevant channel biology settings makes it suitable for a range of dynamically reversible photoswitching studies in TRP channel biology, with the aim to decipher the various biological roles of this centrally important ion channel.

Citing Articles

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Detour to success: photoswitching indirect excitation.

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BTDAzo: A Photoswitchable TRPC5 Channel Activator.

Muller M, Niemeyer K, Urban N, Ojha N, Zufall F, Leinders-Zufall T Angew Chem Int Ed Engl. 2022; 61(36):e202201565.

PMID: 35713469 PMC: 9542918. DOI: 10.1002/anie.202201565.

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