The Spread of Insertion Sequences Element and Transposons in Carbapenem Resistant in a Hospital Setting in Southwestern Iran

Overview

Journal Infect Chemother

Specialty Infectious Diseases

Date 2022 Jun 15

PMID 35706082

Authors

Zahra Hashemizadeh

Gholamreza Hatam

Javad Fathi

Fatemeh Aminazadeh

Hossein Hosseini-Nave

Mahtab Hadadi

Nafiseh Hosseinzadeh Shakib

Sodeh Kholdi

Abdollah Bazargani

Affiliations

Soon will be listed here.

Abstract

Background: is one of the most important hospital pathogenic bacteria that cause infectious diseases. The present study aimed to determine the frequency of carbapenem resistance genes in association with transposable elements and molecular typing of carbapenem-resistant bacteria collected from patients in Shiraz, Iran.

Materials And Methods: A total of 170 carbapenem-resistant isolates were obtained from different clinical specimens in two hospitals. The minimum inhibitory concentrations (MIC) of imipenem were determined and the prevalence of OXA Carbapenemases, Metallo-β-lactamases genes, insertion sequences (IS) elements, and transposons were evaluated by the polymerase chain reaction (PCR) method. Finally, molecular typing of the isolates was performed by the Enterobacterial Repetitive Intergenic Consensus-PCR method.

Results: The MICs ranged from 16 to 1,024 µg/mL for imipenem-resistant isolates. Out of the 170 carbapenem resistant isolates, (94, 55.3%) followed by (71, 41.7%) were predominant. In addition, isolates carried (71, 41.7%), (32, 18.8%), (4, 2.3%), and (1, 0.6%). Moreover, IS (94.2%) and Tn2009 (39.2%) were the most frequent transposable elements. Furthermore, (71, 44.0%) and (161, 94.7%) of the IS of the isolates were associated with and genes, respectively. Besides (3, 1.7%), (1, 0.6%) and (5, 2.9%) of were associated with IS, IS, and IS, respectively. Considering an 80.0% cut off, clusters and four singletons were detected.

Conclusion: According to the results, transposable elements played an important role in the development of resistance genes and resistance to carbapenems. The results also indicated carbapenem-resistant bacteria as a public health concern.

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