Simultaneous Quantitative Analysis of the Major Bioactive Compounds in and Its Beverages by UHPSFC-DAD

Overview

Journal J Agric Food Chem

Specialties Chemistry
Nutritional Sciences

Date 2022 Jun 13

PMID 35695390

Authors

Nora Gibitz-Eisath

Christoph Seger

Stefan Schwaiger

Sonja Sturm

Hermann Stuppner

Affiliations

Soon will be listed here.

Abstract

This study presents the first ultra-high performance supercritical fluid chromatography-diode array detector (UHPSFC-DAD) assay for simultaneous quantitation of secoiridoids, iridoids, xanthones, and xanthone glycosides in L. Separation was reached within 12 min on an Acquity UPC BEH 2-EP column using CO and methanol with 5.5% water as mobile phases. Method validation for nine selected marker compounds (gentisin, isogentisin, swertiamarin, sweroside, gentiopicroside, loganic acid, amarogentin, gentioside, and its isomer) confirmed the assay's sensitivity, linearity, precision, and accuracy. The practical applicability was proven by the analysis of 13 root specimens and 10 commercial liquid preparations (seven liqueurs and three clear spirits). In all root batches, the secoiridoid gentiopicroside dominated (2.1-5.6%) clearly over all other metabolites. In the liqueurs, the metabolite content and distribution were extremely variable: while gentiopicroside was the main compound in four liqueurs, sweroside dominated in one preparation and loganic acid in two others. In contrast, measurable amounts of the metabolites were not detected in any of the examined clear spirits.

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