Beneficial Effect of HS-Releasing Molecules in an In Vitro Model of Sarcopenia: Relevance of Glucoraphanin

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Jun 10

PMID 35682634

Authors

Laura Micheli

Emma Mitidieri

Carlotta Turnaturi

Domenico Vanacore

Clara Ciampi

Elena Lucarini

Giuseppe Cirino

Carla Ghelardini

Raffaella Sorrentino

Lorenzo Di Cesare Mannelli

Roberta dEmmanuele di Villa Bianca

Affiliations

Soon will be listed here.

Abstract

Sarcopenia is a gradual and generalized skeletal muscle (SKM) syndrome, characterized by the impairment of muscle components and functionality. Hydrogen sulfide (HS), endogenously formed within the body from the activity of cystathionine-γ-lyase (CSE), cystathionine- β-synthase (CBS), and mercaptopyruvate sulfurtransferase, is involved in SKM function. Here, in an in vitro model of sarcopenia based on damage induced by dexamethasone (DEX, 1 μM, 48 h treatment) in C2C12-derived myotubes, we investigated the protective potential of exogenous and endogenous sources of HS, i.e., glucoraphanin (30 μM), L-cysteine (150 μM), and 3-mercaptopyruvate (150 μM). DEX impaired the HS signalling in terms of a reduction in CBS and CSE expression and HS biosynthesis. Glucoraphanin and 3-mercaptopyruvate but not L-cysteine prevented the apoptotic process induced by DEX. In parallel, the HS-releasing molecules reduced the oxidative unbalance evoked by DEX, reducing catalase activity, O levels, and protein carbonylation. Glucoraphanin, 3-mercaptopyruvate, and L-cysteine avoided the changes in myotubes morphology and morphometrics after DEX treatment. In conclusion, in an in vitro model of sarcopenia, an impairment in CBS/CSE/HS signalling occurs, whereas glucoraphanin, a natural HS-releasing molecule, appears more effective for preventing the SKM damage. Therefore, glucoraphanin supplementation could be an innovative therapeutic approach in the management of sarcopenia.

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