Proteomic Analysis of Lung Cancer Types-A Pilot Study

Overview

Journal Cancers (Basel)

Publisher MDPI

Specialty Oncology

Date 2022 Jun 10

PMID 35681609

Authors

Simon Sugar

Fanni Bugyi

Gabor Toth

Judit Papay

Ilona Kovalszky

Tamas Tornoczky

Laszlo Drahos

Lilla Turiak

Affiliations

Soon will be listed here.

Abstract

Lung cancer is the leading cause of tumor-related mortality, therefore significant effort is directed towards understanding molecular alterations occurring at the origin of the disease to improve current treatment options. The aim of our pilot-scale study was to carry out a detailed proteomic analysis of formalin-fixed paraffin-embedded tissue sections from patients with small cell or non-small cell lung cancer (adenocarcinoma, squamous cell carcinoma, and large cell carcinoma). Tissue surface digestion was performed on relatively small cancerous and tumor-adjacent normal regions and differentially expressed proteins were identified using label-free quantitative mass spectrometry and subsequent statistical analysis. Principal component analysis clearly distinguished cancerous and cancer adjacent normal samples, while the four lung cancer types investigated had distinct molecular profiles and gene set enrichment analysis revealed specific dysregulated biological processes as well. Furthermore, proteins with altered expression unique to a specific lung cancer type were identified and could be the targets of future studies.

Citing Articles

Lung Cancer Subtyping: A Short Review.

Siddique F, Shehata M, Ghazal M, Contractor S, El-Baz A Cancers (Basel). 2024; 16(15).

PMID: 39123371 PMC: 11312171. DOI: 10.3390/cancers16152643.

Proteomic Profiling of Small-Cell Lung Cancer: A Systematic Review.

Elshoeibi A, Elsayed B, Kaleem M, Elhadary M, Abu-Haweeleh M, Haithm Y Cancers (Basel). 2023; 15(20).

PMID: 37894372 PMC: 10605593. DOI: 10.3390/cancers15205005.

Supervised topological data analysis for MALDI mass spectrometry imaging applications.

Klaila G, Vutov V, Stefanou A BMC Bioinformatics. 2023; 24(1):279.

PMID: 37430224 PMC: 10334630. DOI: 10.1186/s12859-023-05402-0.

Whole-exome and targeted gene sequencing of large-cell lung carcinoma reveals recurrent mutations in the PI3K pathway.

Guo J, Ma Y, Lin J, Jiang G, He J, Lu H Br J Cancer. 2023; 129(2):366-373.

PMID: 37179440 PMC: 10338432. DOI: 10.1038/s41416-023-02301-2.

Compositional Analysis of Glycosaminoglycans in Different Lung Cancer Types-A Pilot Study.

Pal D, Toth G, Sugar S, Fugedi K, Szabo D, Kovalszky I Int J Mol Sci. 2023; 24(8).

PMID: 37108213 PMC: 10138872. DOI: 10.3390/ijms24087050.

References

Wun T, White R . Epidemiology of cancer-related venous thromboembolism. Best Pract Res Clin Haematol. 2009; 22(1):9-23. PMC: 2702321. DOI: 10.1016/j.beha.2008.12.001. View

Fang S, Dai Y, Mei Y, Yang M, Hu L, Yang H . Clinical significance and biological role of cancer-derived Type I collagen in lung and esophageal cancers. Thorac Cancer. 2019; 10(2):277-288. PMC: 6360244. DOI: 10.1111/1759-7714.12947. View

Sung H, Ferlay J, Siegel R, Laversanne M, Soerjomataram I, Jemal A . Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021; 71(3):209-249. DOI: 10.3322/caac.21660. View

Desterro J, Bak-Gordon P, Carmo-Fonseca M . Targeting mRNA processing as an anticancer strategy. Nat Rev Drug Discov. 2019; 19(2):112-129. DOI: 10.1038/s41573-019-0042-3. View

Tanjore H, Kalluri R . The role of type IV collagen and basement membranes in cancer progression and metastasis. Am J Pathol. 2006; 168(3):715-7. PMC: 1606530. DOI: 10.2353/ajpath.2006.051321. View

Pan J, Chen H, Sun Y, Zhang J, Luo X . Comparative proteomic analysis of non-small-cell lung cancer and normal controls using serum label-free quantitative shotgun technology. Lung. 2008; 186(4):255-261. DOI: 10.1007/s00408-008-9093-7. View

Xu J, Zhang C, Wang X, Zhai L, Ma Y, Mao Y . Integrative Proteomic Characterization of Human Lung Adenocarcinoma. Cell. 2020; 182(1):245-261.e17. DOI: 10.1016/j.cell.2020.05.043. View

Jiang C, Huang T, Wang Y, Huang G, Wan X, Gu J . Immunoglobulin G expression in lung cancer and its effects on metastasis. PLoS One. 2014; 9(5):e97359. PMC: 4031068. DOI: 10.1371/journal.pone.0097359. View

Maziveyi M, Alahari S . Cell matrix adhesions in cancer: The proteins that form the glue. Oncotarget. 2017; 8(29):48471-48487. PMC: 5564663. DOI: 10.18632/oncotarget.17265. View

10.

Willumsen N, Bager C, Leeming D, Bay-Jensen A, Karsdal M . Nidogen-1 Degraded by Cathepsin S can be Quantified in Serum and is Associated with Non-Small Cell Lung Cancer. Neoplasia. 2017; 19(4):271-278. PMC: 5344320. DOI: 10.1016/j.neo.2017.01.008. View

11.

Su C, Li J, Zhang L, Wang H, Wang F, Tao Y . The Biological Functions and Clinical Applications of Integrins in Cancers. Front Pharmacol. 2020; 11:579068. PMC: 7522798. DOI: 10.3389/fphar.2020.579068. View

12.

Chang L, Fang S, Gu W . The Molecular Mechanism of Metabolic Remodeling in Lung Cancer. J Cancer. 2020; 11(6):1403-1411. PMC: 6995370. DOI: 10.7150/jca.31406. View

13.

Hatzivassiliou G, Zhao F, Bauer D, Andreadis C, Shaw A, Dhanak D . ATP citrate lyase inhibition can suppress tumor cell growth. Cancer Cell. 2005; 8(4):311-21. DOI: 10.1016/j.ccr.2005.09.008. View

14.

Birse C, Lagier R, Fitzhugh W, Pass H, Rom W, Edell E . Blood-based lung cancer biomarkers identified through proteomic discovery in cancer tissues, cell lines and conditioned medium. Clin Proteomics. 2015; 12(1):18. PMC: 4537594. DOI: 10.1186/s12014-015-9090-9. View

15.

Tsuna M, Kageyama S, Fukuoka J, Kitano H, Doki Y, Tezuka H . Significance of S100A4 as a prognostic marker of lung squamous cell carcinoma. Anticancer Res. 2009; 29(7):2547-54. View

16.

Inamura K . Lung Cancer: Understanding Its Molecular Pathology and the 2015 WHO Classification. Front Oncol. 2017; 7:193. PMC: 5581350. DOI: 10.3389/fonc.2017.00193. View

17.

Wei J, Hu M, Huang K, Lin S, Du H . Roles of Proteoglycans and Glycosaminoglycans in Cancer Development and Progression. Int J Mol Sci. 2020; 21(17). PMC: 7504257. DOI: 10.3390/ijms21175983. View

18.

Calabrese F, Lunardi F, Pezzuto F, Fortarezza F, Vuljan S, Marquette C . Are There New Biomarkers in Tissue and Liquid Biopsies for the Early Detection of Non-Small Cell Lung Cancer?. J Clin Med. 2019; 8(3). PMC: 6463117. DOI: 10.3390/jcm8030414. View

19.

Teng Y, Wang Z, Ma L, Zhang L, Guo Y, Gu M . Prognostic significance of circulating laminin gamma2 for early-stage non-small-cell lung cancer. Onco Targets Ther. 2016; 9:4151-62. PMC: 4939988. DOI: 10.2147/OTT.S105732. View

20.

Hanahan D, Weinberg R . Hallmarks of cancer: the next generation. Cell. 2011; 144(5):646-74. DOI: 10.1016/j.cell.2011.02.013. View