Antigenotoxic Effect of Ascorbic Acid and Resveratrol in Erythrocytes of , and Human Lymphocytes Exposed to Glyphosate

Overview

Journal Curr Issues Mol Biol

Publisher MDPI

Specialty Molecular Biology

Date 2022 Jun 9

PMID 35678680

Authors

Carlos Alvarez-Moya

Alexis Gerardo Samano-Leon

Monica Reynoso-Silva

Rafael Ramirez-Velasco

Mario Alberto Ruiz-Lopez

Alma Rosa Villalobos-Arambula

Affiliations

Soon will be listed here.

Abstract

Glyphosate is a controversial herbicide. Its genotoxicity and presence in various ecosystems have been reported. The use of ascorbic acid and resveratrol could protect different organisms from glyphosate-induced genetic damage. In the present study, specific genetic damage induced by glyphosate was evaluated in erythrocytes of Oreochromis niloticus, Ambystoma mexicanum and human lymphocytes. Simultaneously, the antigenotoxic capacity of various concentrations of ascorbic acid and resveratrol was evaluated by means of pretreatment and simultaneous treatment protocols. The 0.03, 0.05 and 0.07 mM concentrations of glyphosate induced significant genotoxic activity (p < 0.05) in human lymphocytes and in erythrocytes of the species studied, and could cause genomic instability in these populations. The reduction in genetic damage observed in human lymphocytes exposed to high concentrations of glyphosate is only apparent: excessive genetic damage was associated with undetectable excessive tail migration length. A significant (p < 0.05) antigenotoxic effect of ascorbic acid and resveratrol was observed in all concentrations, organisms and protocols used. Both ascorbic acid and resveratrol play an important role in maintaining the integrity of DNA. Ascorbic acid in Oreochromis niloticus, Ambystoma mexicanum reduced glyphosate-induced genetic damage to a basal level. Therefore, our data indicate that these antioxidants could help preserve the integrity of the DNA of organisms exposed to glyphosate. The consumption of antioxidants is a useful tool against the genotoxicity of glyphosate.

Citing Articles

Towards Protection of Nucleic Acids from Herbicide Attack: Self-Assembly of Betaines Based on Pillar[5]arene with Glyphosate and DNA.

Nazarova A, Padnya P, Khannanov A, Khabibrakhmanova A, Zelenikhin P, Stoikov I Int J Mol Sci. 2023; 24(9).

PMID: 37176066 PMC: 10179701. DOI: 10.3390/ijms24098357.

Assessment of Genetic Damage Induced via Glyphosate and Three Commercial Formulations with Adjuvants in Human Blood Cells.

Alvarez-Moya C, Reynoso-Silva M Int J Mol Sci. 2023; 24(5).

PMID: 36901992 PMC: 10003414. DOI: 10.3390/ijms24054560.

References

Dal Santo G, Grotto A, Boligon A, da Costa B, Rambo C, Fantini E . Protective effect of Uncaria tomentosa extract against oxidative stress and genotoxicity induced by glyphosate-Roundup® using zebrafish (Danio rerio) as a model. Environ Sci Pollut Res Int. 2018; 25(12):11703-11715. DOI: 10.1007/s11356-018-1350-6. View

Soloneski S, Ruiz de Arcaute C, Larramendy M . Genotoxic effect of a binary mixture of dicamba- and glyphosate-based commercial herbicide formulations on Rhinella arenarum (Hensel, 1867) (Anura, Bufonidae) late-stage larvae. Environ Sci Pollut Res Int. 2016; 23(17):17811-21. DOI: 10.1007/s11356-016-6992-7. View

Tsai W . Trends in the Use of Glyphosate Herbicide and Its Relevant Regulations in Taiwan: A Water Contaminant of Increasing Concern. Toxics. 2019; 7(1). PMC: 6468642. DOI: 10.3390/toxics7010004. View

Almeida I, Dusman E, Mattge G, Toledo F, Reusing A, Vicentini V . In vivo antimutagenic activity of the medicinal plants Pfaffia glomerata (Brazilian ginseng) and Ginkgo biloba. Genet Mol Res. 2017; 16(3). DOI: 10.4238/gmr16039785. View

Nagy K, Tessema R, Budnik L, Adam B . Comparative cyto- and genotoxicity assessment of glyphosate and glyphosate-based herbicides in human peripheral white blood cells. Environ Res. 2019; 179(Pt B):108851. DOI: 10.1016/j.envres.2019.108851. View

Alvarez-Moya C, Reynoso Silva M, Ramirez C, Gallardo D, Sanchez R, Aguirre A . Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms. Genet Mol Biol. 2014; 37(1):105-10. PMC: 3958316. DOI: 10.1590/s1415-47572014000100016. View

Rossi L, Luaces J, Palermo A, Merani M, Mudry M . Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup). Ecotoxicol Environ Saf. 2018; 157:121-127. DOI: 10.1016/j.ecoenv.2018.03.046. View

Yim S, Lee J, Jo H, Scholten J, Willingham R, Nicoll J . Chrysanthemum Morifolium Extract And Ascorbic Acid-2-Glucoside (AA2G) Blend Inhibits UVA-Induced Delayed Cyclobutane Pyrimidine Dimer (CPD) Production In Melanocytes. Clin Cosmet Investig Dermatol. 2020; 12:823-832. PMC: 6859469. DOI: 10.2147/CCID.S223802. View

Hosseini Bai S, Ogbourne S . Glyphosate: environmental contamination, toxicity and potential risks to human health via food contamination. Environ Sci Pollut Res Int. 2016; 23(19):18988-9001. DOI: 10.1007/s11356-016-7425-3. View

10.

Oliveira R, Ribeiro L, da Silva A, Matuo R, Mantovani M . Evaluation of antimutagenic activity and mechanisms of action of beta-glucan from barley, in CHO-k1 and HTC cell lines using the micronucleus test. Toxicol In Vitro. 2006; 20(7):1225-33. DOI: 10.1016/j.tiv.2006.04.001. View

11.

Milesi M, Lorenz V, Pacini G, Repetti M, Demonte L, Varayoud J . Perinatal exposure to a glyphosate-based herbicide impairs female reproductive outcomes and induces second-generation adverse effects in Wistar rats. Arch Toxicol. 2018; 92(8):2629-2643. DOI: 10.1007/s00204-018-2236-6. View

12.

Andreotti G, Koutros S, Hofmann J, Sandler D, Lubin J, Lynch C . Glyphosate Use and Cancer Incidence in the Agricultural Health Study. J Natl Cancer Inst. 2017; 110(5):509-516. PMC: 6279255. DOI: 10.1093/jnci/djx233. View

13.

Townsend M, Peck C, Meng W, Heaton M, Robison R, ONeill K . Evaluation of various glyphosate concentrations on DNA damage in human Raji cells and its impact on cytotoxicity. Regul Toxicol Pharmacol. 2017; 85:79-85. DOI: 10.1016/j.yrtph.2017.02.002. View

14.

Milic M, Zunec S, Micek V, Kasuba V, Mikolic A, Tariba Lovakovic B . Oxidative stress, cholinesterase activity, and DNA damage in the liver, whole blood, and plasma of Wistar rats following a 28-day exposure to glyphosate. Arh Hig Rada Toksikol. 2018; 69(2):154-168. DOI: 10.2478/aiht-2018-69-3114. View

15.

Schaumburg L, Siroski P, Poletta G, Mudry M . Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos. Pestic Biochem Physiol. 2016; 130:71-78. DOI: 10.1016/j.pestbp.2015.11.009. View

16.

Cavusoglu K, Yapar K, Oruc E, Yalcin E . Protective effect of Ginkgo biloba L. leaf extract against glyphosate toxicity in Swiss albino mice. J Med Food. 2011; 14(10):1263-72. DOI: 10.1089/jmf.2010.0202. View

17.

Jia R, Li Y, Cao L, Du J, Zheng T, Qian H . Antioxidative, anti-inflammatory and hepatoprotective effects of resveratrol on oxidative stress-induced liver damage in tilapia (Oreochromis niloticus). Comp Biochem Physiol C Toxicol Pharmacol. 2018; 215:56-66. DOI: 10.1016/j.cbpc.2018.10.002. View

18.

Romero-Jimenez M, Campos-Sanchez J, Analla M, Munoz-Serrano A, Alonso-Moraga A . Genotoxicity and anti-genotoxicity of some traditional medicinal herbs. Mutat Res. 2005; 585(1-2):147-55. DOI: 10.1016/j.mrgentox.2005.05.004. View

19.

Poletta G, Larriera A, Kleinsorge E, Mudry M . Genotoxicity of the herbicide formulation Roundup (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test. Mutat Res. 2008; 672(2):95-102. DOI: 10.1016/j.mrgentox.2008.10.007. View

20.

Herek J, Vargas L, Rinas Trindade S, Rutkoski C, Macagnan N, Hartmann P . Genotoxic effects of glyphosate on Physalaemus tadpoles. Environ Toxicol Pharmacol. 2020; 81:103516. DOI: 10.1016/j.etap.2020.103516. View