Control of Biofilm-producing Pseudomonas Aeruginosa Isolated from Dairy Farm Using Virokill Silver Nano-based Disinfectant As an Alternative Approach

Overview

Journal Sci Rep

Specialty Science

Date 2022 Jun 8

PMID 35676412

Authors

Sahar Abdel Aleem Abdel Aziz

Rehab Mahmoud

Manar Bahaa El Din Mohamed

Affiliations

Soon will be listed here.

Abstract

Pseudomonas aeruginosa (P. aeruginosa) is an important opportunistic pathogen that is responsible for many clinical infections in both animals and humans. This study aimed to detect the prevalence of P. aeruginosa in dairy farm's that possess a great importance to dairy industry where it shares in milk spoilage. Evaluation of the efficacy of commonly used disinfectants to control the pathogen in dairy environment and finding a way to overcome high resistance to the used agents. Samples (n = 250) were collected from different environmental components, milk, and milkers' hands. Pathogens were isolated, biofilm was detected and their sensitivity against two commonly used disinfectants and against silver nanoparticles and Virokill AgNPs at different concentrations and contact times were tested. The pathogen significantly prevailed in milk samples (70.0%, P < 0.001). 50 out 74 isolates were biofilm-forming that was significantly obtained from environment (71.8%, P < 0.001). P. aeruginosa showed variable degree of resistance to tested disinfectants but it was significantly sensitive to Virokill AgNPs (200/1000) mg/l at exposure time 24 h (P < 0.001). It was concluded that using Virokill AgNPs in regular sanitation and disinfection of dairy farms, this helps the control of P. aeruginosa subsequently increasing milk quality and improving dairy industry and protecting human health.

Citing Articles

Does Every Strain of Attack the Same? Results of a Study of the Prevalence of Virulence Factors of Strains Obtained from Different Animal Species in Northeastern Poland.

Foksinski P, Blank A, Kaczorek-Lukowska E, Malaczewska J, Wrobel M, Wojcik E Pathogens. 2024; 13(11).

PMID: 39599532 PMC: 11597259. DOI: 10.3390/pathogens13110979.

Psychrotrophic Bacteria Threatening the Safety of Animal-Derived Foods: Characteristics, Contamination, and Control Strategies.

Oh H, Lee J Food Sci Anim Resour. 2024; 44(5):1011-1027.

PMID: 39246535 PMC: 11377203. DOI: 10.5851/kosfa.2024.e70.

Prevalence of Multidrug-Resistant Isolated from Dairy Cattle, Milk, Environment, and Workers' Hands.

Badawy B, Moustafa S, Shata R, Sayed-Ahmed M, Alqahtani S, Ali M Microorganisms. 2023; 11(11).

PMID: 38004786 PMC: 10672731. DOI: 10.3390/microorganisms11112775.

References

Abdallah M, Benoliel C, Drider D, Dhulster P, Chihib N . Biofilm formation and persistence on abiotic surfaces in the context of food and medical environments. Arch Microbiol. 2014; 196(7):453-72. DOI: 10.1007/s00203-014-0983-1. View

Campodonico V, Llosa N, Grout M, Doring G, Maira-Litran T, Pier G . Evaluation of flagella and flagellin of Pseudomonas aeruginosa as vaccines. Infect Immun. 2009; 78(2):746-55. PMC: 2812208. DOI: 10.1128/IAI.00806-09. View

Li Q, Mahendra S, Lyon D, Brunet L, Liga M, Li D . Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications. Water Res. 2008; 42(18):4591-602. DOI: 10.1016/j.watres.2008.08.015. View

Drenkard E . Antimicrobial resistance of Pseudomonas aeruginosa biofilms. Microbes Infect. 2003; 5(13):1213-9. DOI: 10.1016/j.micinf.2003.08.009. View

Munoz M, Zadoks R . Short communication: Patterns of fecal shedding of Klebsiella by dairy cows. J Dairy Sci. 2007; 90(3):1220-4. DOI: 10.3168/jds.S0022-0302(07)71610-7. View

Govarthanan M, Selvankumar T, Manoharan K, Rathika R, Shanthi K, Lee K . Biosynthesis and characterization of silver nanoparticles using panchakavya, an Indian traditional farming formulating agent. Int J Nanomedicine. 2014; 9:1593-9. PMC: 3983015. DOI: 10.2147/IJN.S58932. View

Stewart P, Rayner J, Roe F, Rees W . Biofilm penetration and disinfection efficacy of alkaline hypochlorite and chlorosulfamates. J Appl Microbiol. 2001; 91(3):525-32. DOI: 10.1046/j.1365-2672.2001.01413.x. View

Blanc D, Petignat C, Janin B, Bille J, Francioli P . Frequency and molecular diversity of Pseudomonas aeruginosa upon admission and during hospitalization: a prospective epidemiologic study. Clin Microbiol Infect. 2002; 4(5):242-247. DOI: 10.1111/j.1469-0691.1998.tb00051.x. View

Bjarnsholt T, Jensen P, Fiandaca M, Pedersen J, Hansen C, Andersen C . Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients. Pediatr Pulmonol. 2009; 44(6):547-58. DOI: 10.1002/ppul.21011. View

10.

Moradali M, Ghods S, Rehm B . Lifestyle: A Paradigm for Adaptation, Survival, and Persistence. Front Cell Infect Microbiol. 2017; 7:39. PMC: 5310132. DOI: 10.3389/fcimb.2017.00039. View

11.

Harada K, Arima S, Niina A, Kataoka Y, Takahashi T . Characterization of Pseudomonas aeruginosa isolates from dogs and cats in Japan: current status of antimicrobial resistance and prevailing resistance mechanisms. Microbiol Immunol. 2011; 56(2):123-7. DOI: 10.1111/j.1348-0421.2011.00416.x. View

12.

Milivojevic D, Sumonja N, Medic S, Pavic A, Moric I, Vasiljevic B . Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans. Pathog Dis. 2018; 76(4). DOI: 10.1093/femspd/fty041. View

13.

Deshmukh S, Patil S, Mullani S, Delekar S . Silver nanoparticles as an effective disinfectant: A review. Mater Sci Eng C Mater Biol Appl. 2019; 97:954-965. PMC: 7127744. DOI: 10.1016/j.msec.2018.12.102. View

14.

Silby M, Winstanley C, Godfrey S, Levy S, Jackson R . Pseudomonas genomes: diverse and adaptable. FEMS Microbiol Rev. 2011; 35(4):652-80. DOI: 10.1111/j.1574-6976.2011.00269.x. View

15.

Gupta A, Silver S . Silver as a biocide: will resistance become a problem?. Nat Biotechnol. 1998; 16(10):888. DOI: 10.1038/nbt1098-888. View

16.

Dua K, Shukla S, Tekade R, Hansbro P . Whether a novel drug delivery system can overcome the problem of biofilms in respiratory diseases?. Drug Deliv Transl Res. 2016; 7(1):179-187. DOI: 10.1007/s13346-016-0349-0. View

17.

Malek A, Wargo M, Hogan D . Absence of membrane phosphatidylcholine does not affect virulence and stress tolerance phenotypes in the opportunistic pathogen Pseudomonas aeruginosa. PLoS One. 2012; 7(2):e30829. PMC: 3281885. DOI: 10.1371/journal.pone.0030829. View

18.

Meyer B, Cookson B . Does microbial resistance or adaptation to biocides create a hazard in infection prevention and control?. J Hosp Infect. 2010; 76(3):200-5. DOI: 10.1016/j.jhin.2010.05.020. View

19.

Sela S, Hammer-Muntz O, Krifucks O, Pinto R, Weisblit L, Leitner G . Phenotypic and genotypic characterization of Pseudomonas aeruginosa strains isolated from mastitis outbreaks in dairy herds. J Dairy Res. 2007; 74(4):425-9. DOI: 10.1017/S0022029907002610. View

20.

Chen W, Zhang Y, Zhang Y, Pi Y, Gu T, Song L . CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species. iScience. 2018; 6:222-231. PMC: 6137401. DOI: 10.1016/j.isci.2018.07.024. View