An Electrochemical Membrane-based Aptasensor for Detection of Severe Acute Respiratory Syndrome Coronavirus-2 Receptor-binding Domain

Overview

Journal Appl Surf Sci

Date 2022 Jun 7

PMID 35669218

Authors

Mahmoud Amouzadeh Tabrizi

Pablo Acedo

Affiliations

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Abstract

Herein, we report an electrochemical membrane-based aptasensor for the determination of the SARS-CoV-2 receptor-binding domain (SARS-CoV-2-RBD). For this purpose, the nanoporous anodic aluminium oxide membrane (NPAOM) was first fabricated electrochemically. The NPAOM was then functionalized with 3-mercaptopropyl trimethoxysilane (NPAOM-Si-SH). After that, the NPAOM-Si-SH was decorated with gold nanoparticles by using gold ion and sodium borohydride. The NPAOM-Si-S-Au was then attached to the surface of the working electrode of a laser-engraved graphene electrode (LEGE). Subsequently, the LEGE/NPAOM-Si-S-Au was fixed inside a flow cell that was made by using a three-dimensional (3D) printer, and then thiolated aptamer was transferred into the flow cell using a pump. The electrochemical behavior of the LEGE/NPAOM-Si-S-Au-Aptamer was studied using square wave voltammetry (SWV) in the presence of potassium ferrocyanide as a redox probe. The response of the LEGE/NPAOM-Si-S-Au-Aptamer to the different concentrations of the SARS-CoV-2-RBD in human saliva sample was investigated in the concentration range of 2.5-40.0 ng/mL. The limit of the detection was found to be 0.8 ng/mL. The LEGE/NPAOM-Si-S-Au-Aptamer showed good selectivity to 5.0 ng/mL of SARS-CoV-2-RBD in the presence of five times of the interfering agents like hemagglutinin and neuraminidase as the influenza A virus major surface glycoproteins.

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References

Bachmann M, Mohsen M, Zha L, Vogel M, Speiser D . SARS-CoV-2 structural features may explain limited neutralizing-antibody responses. NPJ Vaccines. 2021; 6(1):2. PMC: 7782831. DOI: 10.1038/s41541-020-00264-6. View

Settu K, Chiu P, Huang Y . Laser-Induced Graphene-Based Enzymatic Biosensor for Glucose Detection. Polymers (Basel). 2021; 13(16). PMC: 8400493. DOI: 10.3390/polym13162795. View

Cutler D, Summers L . The COVID-19 Pandemic and the $16 Trillion Virus. JAMA. 2020; 324(15):1495-1496. PMC: 7604733. DOI: 10.1001/jama.2020.19759. View

Talebian S, Wallace G, Schroeder A, Stellacci F, Conde J . Nanotechnology-based disinfectants and sensors for SARS-CoV-2. Nat Nanotechnol. 2020; 15(8):618-621. DOI: 10.1038/s41565-020-0751-0. View

de Carvalho A, Pilling M, Gardner P, Doherty J, Cinque G, Wehbe K . Chemotherapeutic response to cisplatin-like drugs in human breast cancer cells probed by vibrational microspectroscopy. Faraday Discuss. 2016; 187:273-98. DOI: 10.1039/c5fd00148j. View

Lee J, Choi M, Jung Y, Lee S, Lee C, Kim J . A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2). Biosens Bioelectron. 2020; 171:112715. PMC: 7560266. DOI: 10.1016/j.bios.2020.112715. View

Eissa S, Alhadrami H, Al-Mozaini M, Hassan A, Zourob M . Voltammetric-based immunosensor for the detection of SARS-CoV-2 nucleocapsid antigen. Mikrochim Acta. 2021; 188(6):199. PMC: 8153846. DOI: 10.1007/s00604-021-04867-1. View

Espinoza-Castaneda M, Escosura-Muniz A, Chamorro A, de Torres C, Merkoci A . Nanochannel array device operating through Prussian blue nanoparticles for sensitive label-free immunodetection of a cancer biomarker. Biosens Bioelectron. 2014; 67:107-14. DOI: 10.1016/j.bios.2014.07.039. View

Roberts A, Mahari S, Shahdeo D, Gandhi S . Label-free detection of SARS-CoV-2 Spike S1 antigen triggered by electroactive gold nanoparticles on antibody coated fluorine-doped tin oxide (FTO) electrode. Anal Chim Acta. 2021; 1188:339207. PMC: 8529383. DOI: 10.1016/j.aca.2021.339207. View

10.

Lasserre P, Balansethupathy B, Vezza V, Butterworth A, Macdonald A, Blair E . SARS-CoV-2 Aptasensors Based on Electrochemical Impedance Spectroscopy and Low-Cost Gold Electrode Substrates. Anal Chem. 2022; 94(4):2126-2133. PMC: 8790822. DOI: 10.1021/acs.analchem.1c04456. View

11.

Fabiani L, Saroglia M, Galata G, De Santis R, Fillo S, Luca V . Magnetic beads combined with carbon black-based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS-CoV-2 detection in saliva. Biosens Bioelectron. 2020; 171:112686. PMC: 7833515. DOI: 10.1016/j.bios.2020.112686. View

12.

Rahmati Z, Roushani M, Hosseini H, Choobin H . Electrochemical immunosensor with CuO nanocube coating for detection of SARS-CoV-2 spike protein. Mikrochim Acta. 2021; 188(3):105. PMC: 7921825. DOI: 10.1007/s00604-021-04762-9. View

13.

Puppels G, Olminkhof J, Segers-Nolten G, Otto C, de Mul F, Greve J . Laser irradiation and Raman spectroscopy of single living cells and chromosomes: sample degradation occurs with 514.5 nm but not with 660 nm laser light. Exp Cell Res. 1991; 195(2):361-7. DOI: 10.1016/0014-4827(91)90385-8. View

14.

Weiss A, Jellingso M, Sommer M . Spatial and temporal dynamics of SARS-CoV-2 in COVID-19 patients: A systematic review and meta-analysis. EBioMedicine. 2020; 58:102916. PMC: 7374142. DOI: 10.1016/j.ebiom.2020.102916. View

15.

Amouzadeh Tabrizi M, Shamsipur M, Farzin L . A high sensitive electrochemical aptasensor for the determination of VEGF(165) in serum of lung cancer patient. Biosens Bioelectron. 2015; 74:764-9. DOI: 10.1016/j.bios.2015.07.032. View

16.

Chaibun T, Puenpa J, Ngamdee T, Boonapatcharoen N, Athamanolap P, OMullane A . Rapid electrochemical detection of coronavirus SARS-CoV-2. Nat Commun. 2021; 12(1):802. PMC: 7864991. DOI: 10.1038/s41467-021-21121-7. View

17.

Yano T, Kajisa T, Ono M, Miyasaka Y, Hasegawa Y, Saito A . Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance. Sci Rep. 2022; 12(1):1060. PMC: 8776812. DOI: 10.1038/s41598-022-05036-x. View

18.

Moitra P, Alafeef M, Dighe K, Frieman M, Pan D . Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles. ACS Nano. 2020; 14(6):7617-7627. DOI: 10.1021/acsnano.0c03822. View

19.

Layqah L, Eissa S . An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes. Mikrochim Acta. 2019; 186(4):224. PMC: 7088225. DOI: 10.1007/s00604-019-3345-5. View

20.

Yousef H, Liu Y, Zheng L . Nanomaterial-Based Label-Free Electrochemical Aptasensors for the Detection of Thrombin. Biosensors (Basel). 2022; 12(4). PMC: 9025199. DOI: 10.3390/bios12040253. View