Cotton MiR319b-Targeted TCP4-Like Enhances Plant Defense Against by Activating Transcription Expression

Overview

Journal Front Plant Sci

Date 2022 Jun 7

PMID 35668804

Authors

Pei Jia

Ye Tang

Guang Hu

Yonggang Quan

Aimin Chen

Naiqin Zhong

Qingzhong Peng

Jiahe Wu

Affiliations

Soon will be listed here.

Abstract

Teosinte branched1/Cincinnata/proliferating cell factor (TCP) transcription factors play important roles in plant growth and defense. However, the molecular mechanisms of TCPs participating in plant defense remain unclear. Here, we characterized a cotton TCP4-like fine-tuned by miR319b, which could interact with NON-EXPRESSER OF PATHOGEN-RELATED GENES 1 (NPR1) to directly activate () expression, facilitating plant resistance against . mRNA degradome data and GUS-fused assay showed that mRNA was directedly cleaved by ghr-miR319b. Knockdown of ghr-miR319b increased plant resistance to , whereas silencing increased plant susceptibility by the virus-induced gene silencing (VIGS) method, suggesting that is a positive regulator of plant defense. According to the electrophoretic mobility shift assay and GUS reporter analysis, GhTCP4-like could transcriptionally activate expression, resulting in increased salicylic acid (SA) accumulation. Yeast two-hybrid and luciferase complementation image analyses demonstrated that interacts with , which can promote transcriptional activation in expression according to the GUS reporter assay. Together, these results revealed that GhTCP4-like interacts with GhNPR1 to promote expression through fine-tuning of ghr-miR319b, leading to SA accumulation, which is percepted by NPR1 to increase plant defense against . Therefore, GhTCP4-like participates in a positive feedback regulation loop of SA biosynthesis via NPR1, increasing plant defenses against fungal infection.

Citing Articles

Cotton Mediates Plant Resistance against by miR482b Regulation.

Wu P, Lu C, Wang B, Zhang F, Shi L, Xu Y Biology (Basel). 2023; 12(7).

PMID: 37508331 PMC: 10376429. DOI: 10.3390/biology12070898.

Interactions between and cotton: pathogenic mechanism and cotton resistance mechanism to Verticillium wilt.

Zhu Y, Zhao M, Li T, Wang L, Liao C, Liu D Front Plant Sci. 2023; 14:1174281.

PMID: 37152175 PMC: 10161258. DOI: 10.3389/fpls.2023.1174281.

Insights to Gossypium defense response against Verticillium dahliae: the Cotton Cancer.

Umer M, Zheng J, Yang M, Batool R, Abro A, Hou Y Funct Integr Genomics. 2023; 23(2):142.

PMID: 37121989 DOI: 10.1007/s10142-023-01065-5.

References

Catinot J, Buchala A, Abou-Mansour E, Metraux J . Salicylic acid production in response to biotic and abiotic stress depends on isochorismate in Nicotiana benthamiana. FEBS Lett. 2008; 582(4):473-8. DOI: 10.1016/j.febslet.2007.12.039. View

Zhang Y, Xu S, Ding P, Wang D, Cheng Y, He J . Control of salicylic acid synthesis and systemic acquired resistance by two members of a plant-specific family of transcription factors. Proc Natl Acad Sci U S A. 2010; 107(42):18220-5. PMC: 2964219. DOI: 10.1073/pnas.1005225107. View

Klimes A, Dobinson K, Thomma B, Klosterman S . Genomics spurs rapid advances in our understanding of the biology of vascular wilt pathogens in the genus Verticillium. Annu Rev Phytopathol. 2015; 53:181-98. DOI: 10.1146/annurev-phyto-080614-120224. View

Liu Y, Guan X, Liu S, Yang M, Ren J, Guo M . Genome-Wide Identification and Analysis of TCP Transcription Factors Involved in the Formation of Leafy Head in Chinese Cabbage. Int J Mol Sci. 2018; 19(3). PMC: 5877708. DOI: 10.3390/ijms19030847. View

Mukhtar M, Carvunis A, Dreze M, Epple P, Steinbrenner J, Moore J . Independently evolved virulence effectors converge onto hubs in a plant immune system network. Science. 2011; 333(6042):596-601. PMC: 3170753. DOI: 10.1126/science.1203659. View

Coquoz , Buchala , Metraux . The biosynthesis of salicylic acid in potato plants . Plant Physiol. 1998; 117(3):1095-101. PMC: 34925. DOI: 10.1104/pp.117.3.1095. View

Nicolas M, Cubas P . TCP factors: new kids on the signaling block. Curr Opin Plant Biol. 2016; 33:33-41. DOI: 10.1016/j.pbi.2016.05.006. View

Zhang T, Hu Y, Jiang W, Fang L, Guan X, Chen J . Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement. Nat Biotechnol. 2015; 33(5):531-7. DOI: 10.1038/nbt.3207. View

Cao H, Bowling S, Gordon A, Dong X . Characterization of an Arabidopsis Mutant That Is Nonresponsive to Inducers of Systemic Acquired Resistance. Plant Cell. 1994; 6(11):1583-1592. PMC: 160545. DOI: 10.1105/tpc.6.11.1583. View

10.

Hu G, Hao M, Wang L, Liu J, Zhang Z, Tang Y . The Cotton miR477- Module Participates in Plant Defense Against . Mol Plant Microbe Interact. 2019; 33(4):624-636. DOI: 10.1094/MPMI-10-19-0302-R. View

11.

Varkonyi-Gasic E, Wu R, Wood M, Walton E, Hellens R . Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs. Plant Methods. 2007; 3:12. PMC: 2225395. DOI: 10.1186/1746-4811-3-12. View

12.

Kosugi S, Ohashi Y . DNA binding and dimerization specificity and potential targets for the TCP protein family. Plant J. 2002; 30(3):337-48. DOI: 10.1046/j.1365-313x.2002.01294.x. View

13.

Nath U, Crawford B, Carpenter R, Coen E . Genetic control of surface curvature. Science. 2003; 299(5611):1404-7. DOI: 10.1126/science.1079354. View

14.

Cao H, Glazebrook J, Clarke J, Volko S, Dong X . The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats. Cell. 1997; 88(1):57-63. DOI: 10.1016/s0092-8674(00)81858-9. View

15.

Wessling R, Epple P, Altmann S, He Y, Yang L, Henz S . Convergent targeting of a common host protein-network by pathogen effectors from three kingdoms of life. Cell Host Microbe. 2014; 16(3):364-75. PMC: 4191710. DOI: 10.1016/j.chom.2014.08.004. View

16.

Gao F, Zhou B, Li G, Jia P, Li H, Zhao Y . A glutamic acid-rich protein identified in Verticillium dahliae from an insertional mutagenesis affects microsclerotial formation and pathogenicity. PLoS One. 2010; 5(12):e15319. PMC: 2998422. DOI: 10.1371/journal.pone.0015319. View

17.

Gao X, Britt Jr R, Shan L, He P . Agrobacterium-mediated virus-induced gene silencing assay in cotton. J Vis Exp. 2011; (54). PMC: 3217629. DOI: 10.3791/2938. View

18.

Zheng X, Spivey N, Zeng W, Liu P, Fu Z, Klessig D . Coronatine promotes Pseudomonas syringae virulence in plants by activating a signaling cascade that inhibits salicylic acid accumulation. Cell Host Microbe. 2012; 11(6):587-96. PMC: 3404825. DOI: 10.1016/j.chom.2012.04.014. View

19.

Van Verk M, Bol J, Linthorst H . WRKY transcription factors involved in activation of SA biosynthesis genes. BMC Plant Biol. 2011; 11:89. PMC: 3120740. DOI: 10.1186/1471-2229-11-89. View

20.

Xiong X, Sun S, Zhu Q, Zhang X, Liu F, Li Y . Transcriptome Analysis and RNA Interference Reveal Regulating Cotton Resistance to Verticillium Wilt by JA and SA Signaling Pathways. Front Plant Sci. 2021; 12:654676. PMC: 8226099. DOI: 10.3389/fpls.2021.654676. View