Control of Salicylic Acid Synthesis and Systemic Acquired Resistance by Two Members of a Plant-specific Family of Transcription Factors

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2010 Oct 6

PMID 20921422

Citations 166

Authors

Yaxi Zhang

Shaohua Xu

Pingtao Ding

Dongmei Wang

Yu Ti Cheng

Jing He

Minghui Gao

Fang Xu

Yan Li

Zhaohai Zhu

Xin Li

Yuelin Zhang

Affiliations

Soon will be listed here.

Abstract

Salicylic acid (SA) is a defense hormone required for both local and systemic acquired resistance (SAR) in plants. Pathogen infections induce SA synthesis through up-regulating the expression of Isochorismate Synthase 1 (ICS1), which encodes a key enzyme in SA production. Here we report that both SAR Deficient 1 (SARD1) and CBP60g are key regulators for ICS1 induction and SA synthesis. Whereas knocking out SARD1 compromises basal resistance and SAR, overexpression of SARD1 constitutively activates defense responses. In the sard1-1 cbp60g-1 double mutant, pathogen-induced ICS1 up-regulation and SA synthesis are blocked in both local and systemic leaves, resulting in compromised basal resistance and loss of SAR. Electrophoretic mobility shift assays showed that SARD1 and CBP60g represent a plant-specific family of DNA-binding proteins. Both proteins are recruited to the promoter of ICS1 in response to pathogen infections, suggesting that they control SA synthesis by regulating ICS1 at the transcriptional level.

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