» Articles » PMID: 35657485

Molecular Analysis of Trypanosome Infections in Algerian Camels

Overview
Journal Acta Parasitol
Specialty Parasitology
Date 2022 Jun 3
PMID 35657485
Authors
Affiliations
Soon will be listed here.
Abstract

Purpose: Surra is an economically important livestock disease in many low- and middle-income countries, including those of Northern Africa. The disease is caused by the biting fly-transmitted subspecies Trypanosoma brucei evansi, which is very closely related to the tsetse-transmitted subspecies T. b. brucei and the sexually transmitted subspecies T. b. equiperdum. At least two phylogenetically distinct groups of T. b. evansi can be distinguished, called type A and type B. These evolved from T. b. brucei independently. The close relationships between the T. brucei subspecies and the multiple evolutionary origins of T. b. evansi pose diagnostic challenges.

Methods: Here we use previously established and newly developed PCR assays based on nuclear and mitochondrial genetic markers to type the causative agent of recent trypanosome infections of camels in Southern Algeria.

Results/conclusion: We confirm that these infections have been caused by T. b. evansi type A. We also report a newly designed PCR assay specific for T. b. evansi type A that we expect will be of diagnostic use for the community.

Citing Articles

Deep kinetoplast genome analyses result in a novel molecular assay for detecting -specific minicircles.

Geerts M, Chen Z, Bebronne N, Savill N, Schnaufer A, Buscher P NAR Genom Bioinform. 2022; 4(4):lqac081.

PMID: 36285287 PMC: 9582789. DOI: 10.1093/nargab/lqac081.

References
1.
Carnes J, Anupama A, Balmer O, Jackson A, Lewis M, Brown R . Genome and phylogenetic analyses of Trypanosoma evansi reveal extensive similarity to T. brucei and multiple independent origins for dyskinetoplasty. PLoS Negl Trop Dis. 2015; 9(1):e3404. PMC: 4288722. DOI: 10.1371/journal.pntd.0003404. View

2.
Cuypers B, Van den Broeck F, Van Reet N, Meehan C, Cauchard J, Wilkes J . Genome-Wide SNP Analysis Reveals Distinct Origins of Trypanosoma evansi and Trypanosoma equiperdum. Genome Biol Evol. 2017; 9(8):1990-1997. PMC: 5566637. DOI: 10.1093/gbe/evx102. View

3.
Claes F, Buscher P, Touratier L, Goddeeris B . Trypanosoma equiperdum: master of disguise or historical mistake?. Trends Parasitol. 2005; 21(7):316-21. DOI: 10.1016/j.pt.2005.05.010. View

4.
Lai D, Hashimi H, Lun Z, Ayala F, Lukes J . Adaptations of Trypanosoma brucei to gradual loss of kinetoplast DNA: Trypanosoma equiperdum and Trypanosoma evansi are petite mutants of T. brucei. Proc Natl Acad Sci U S A. 2008; 105(6):1999-2004. PMC: 2538871. DOI: 10.1073/pnas.0711799105. View

5.
Aregawi W, Agga G, Abdi R, Buscher P . Systematic review and meta-analysis on the global distribution, host range, and prevalence of Trypanosoma evansi. Parasit Vectors. 2019; 12(1):67. PMC: 6357473. DOI: 10.1186/s13071-019-3311-4. View