Murine Brain Tumor Microenvironment Immunophenotyping Using Mass Cytometry
Overview
Overview
Journal
STAR Protoc
Publisher
Cell Press
Specialties
Biology
Biomedical Engineering
Science
Biomedical Engineering
Science
Date
2022 May 31
PMID
35634359
Authors
Affiliations
Affiliations
Soon will be listed here.
Abstract
Here, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model. We describe antibody conjugation and titrations for analysis of immune cells. We then detail mouse brain tumor tissue collection and processing, staining, followed by data acquisition, analysis, and gating strategy. This protocol can be applied to any brain tumor-harboring mouse model. For complete details on the use and execution of this protocol, please refer to Alghamri et al. (2021).
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