Muscle Spindles of the Multifidus Muscle Undergo Structural Change After Intervertebral Disc Degeneration

Overview

Journal Eur Spine J

Specialty Orthopedics

Date 2022 May 26

PMID 35618974

Authors

Gregory James

Carla Stecco

Linda Blomster

Leanne Hall

Annina B Schmid

Cindy C Shu

Christopher B Little

James Melrose

Paul W Hodges

Affiliations

Soon will be listed here.

Abstract

Purpose: Proprioceptive deficits are common in low back pain. The multifidus muscle undergoes substantial structural change after back injury, but whether muscle spindles are affected is unclear. This study investigated whether muscle spindles of the multifidus muscle are changed by intervertebral disc (IVD) degeneration in a large animal model.

Methods: IVD degeneration was induced by partial thickness annulus fibrosus lesion to the L3-4 IVD in nine sheep. Multifidus muscle tissue at L4 was harvested at six months after lesion, and from six age-/sex-matched naïve control animals. Muscle spindles were identified in Van Gieson's-stained sections by morphology. The number, location and cross-sectional area (CSA) of spindles, the number, type and CSA of intrafusal fibers, and thickness of the spindle capsule were measured. Immunofluorescence assays examined Collagen I and III expression.

Results: Multifidus muscle spindles were located centrally in the muscle and generally near connective tissue. There were no differences in the number or location of muscle spindles after IVD degeneration and only changes in the CSA of nuclear chain fibers. The thickness of connective tissue surrounding the muscle spindle was increased as was the expression of Collagen I and III.

Conclusion: Changes to the connective tissue and collagen expression of the muscle spindle capsule are likely to impact their mechanical properties. Changes in capsule stiffness may impact the transmission of length change to muscle spindles and thus transduction of sensory information. This change in muscle spindle structure may explain some of the proprioceptive deficits identified with low back pain.

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References

Mannion A, Weber B, Dvorak J, Grob D, Muntener M . Fibre type characteristics of the lumbar paraspinal muscles in normal healthy subjects and in patients with low back pain. J Orthop Res. 1998; 15(6):881-7. DOI: 10.1002/jor.1100150614. View

Xu Y, Choi J, Reeves N, Cholewicki J . Optimal control of the spine system. J Biomech Eng. 2010; 132(5):051004. DOI: 10.1115/1.4000955. View

Hoy D, March L, Brooks P, Blyth F, Woolf A, Bain C . The global burden of low back pain: estimates from the Global Burden of Disease 2010 study. Ann Rheum Dis. 2014; 73(6):968-74. DOI: 10.1136/annrheumdis-2013-204428. View

Schroder J . The fine structure of de- and reinnervated muscle spindles. II. Regenerated sensory and motor nerve terminals. Acta Neuropathol. 1974; 30(2):129-44. DOI: 10.1007/BF00685438. View

Shahidi B, Hubbard J, Gibbons M, Ruoss S, Zlomislic V, Allen R . Lumbar multifidus muscle degenerates in individuals with chronic degenerative lumbar spine pathology. J Orthop Res. 2017; 35(12):2700-2706. PMC: 5677570. DOI: 10.1002/jor.23597. View

Hodges P, James G, Blomster L, Hall L, Schmid A, Shu C . Multifidus Muscle Changes After Back Injury Are Characterized by Structural Remodeling of Muscle, Adipose and Connective Tissue, but Not Muscle Atrophy: Molecular and Morphological Evidence. Spine (Phila Pa 1976). 2015; 40(14):1057-71. DOI: 10.1097/BRS.0000000000000972. View

James G, Blomster L, Hall L, Schmid A, Shu C, Little C . Mesenchymal Stem Cell Treatment of Intervertebral Disc Lesion Prevents Fatty Infiltration and Fibrosis of the Multifidus Muscle, but not Cytokine and Muscle Fiber Changes. Spine (Phila Pa 1976). 2016; 41(15):1208-1217. DOI: 10.1097/BRS.0000000000001669. View

Brumagne S, Cordo P, Lysens R, Verschueren S, Swinnen S . The role of paraspinal muscle spindles in lumbosacral position sense in individuals with and without low back pain. Spine (Phila Pa 1976). 2000; 25(8):989-94. DOI: 10.1097/00007632-200004150-00015. View

Panjabi M . A hypothesis of chronic back pain: ligament subfailure injuries lead to muscle control dysfunction. Eur Spine J. 2005; 15(5):668-76. PMC: 3489327. DOI: 10.1007/s00586-005-0925-3. View

10.

Moseley G, Hodges P . Reduced variability of postural strategy prevents normalization of motor changes induced by back pain: a risk factor for chronic trouble?. Behav Neurosci. 2006; 120(2):474-476. DOI: 10.1037/0735-7044.120.2.474. View

11.

James G, Klyne D, Millecamps M, Stone L, Hodges P . ISSLS Prize in Basic science 2019: Physical activity attenuates fibrotic alterations to the multifidus muscle associated with intervertebral disc degeneration. Eur Spine J. 2019; 28(5):893-904. DOI: 10.1007/s00586-019-05902-9. View

12.

Hildebrandt M, Fankhauser G, Meichtry A, Luomajoki H . Correlation between lumbar dysfunction and fat infiltration in lumbar multifidus muscles in patients with low back pain. BMC Musculoskelet Disord. 2017; 18(1):12. PMC: 5223418. DOI: 10.1186/s12891-016-1376-1. View

13.

James G, Millecamps M, S Stone L, Hodges P . Multifidus Muscle Fiber Type Distribution is Changed in Mouse Models of Chronic Intervertebral Disc Degeneration, but is not Attenuated by Whole Body Physical Activity. Spine (Phila Pa 1976). 2021; 46(23):1612-1620. DOI: 10.1097/BRS.0000000000004105. View

14.

Thoma A, Lightfoot A . NF-kB and Inflammatory Cytokine Signalling: Role in Skeletal Muscle Atrophy. Adv Exp Med Biol. 2018; 1088:267-279. DOI: 10.1007/978-981-13-1435-3_12. View

15.

Korakakis V, OSullivan K, Kotsifaki A, Sotiralis Y, Giakas G . Lumbo-pelvic proprioception in sitting is impaired in subgroups of low back pain-But the clinical utility of the differences is unclear. A systematic review and meta-analysis. PLoS One. 2021; 16(4):e0250673. PMC: 8075231. DOI: 10.1371/journal.pone.0250673. View

16.

Ovalle W, DOW P, Nahirney P . Structure, distribution and innervation of muscle spindles in avian fast and slow skeletal muscle. J Anat. 1999; 194 ( Pt 3):381-94. PMC: 1467938. DOI: 10.1046/j.1469-7580.1999.19430381.x. View

17.

Melrose J, Shu C, Young C, Ho R, Smith M, Young A . Mechanical destabilization induced by controlled annular incision of the intervertebral disc dysregulates metalloproteinase expression and induces disc degeneration. Spine (Phila Pa 1976). 2011; 37(1):18-25. DOI: 10.1097/BRS.0b013e31820cd8d5. View

18.

Brown S, Gregory D, Carr J, Ward S, Masuda K, Lieber R . ISSLS prize winner: Adaptations to the multifidus muscle in response to experimentally induced intervertebral disc degeneration. Spine (Phila Pa 1976). 2011; 36(21):1728-36. DOI: 10.1097/BRS.0b013e318212b44b. View

19.

Kroger S, Watkins B . Muscle spindle function in healthy and diseased muscle. Skelet Muscle. 2021; 11(1):3. PMC: 7788844. DOI: 10.1186/s13395-020-00258-x. View

20.

Proske U, Tsay A, Allen T . Muscle thixotropy as a tool in the study of proprioception. Exp Brain Res. 2014; 232(11):3397-412. DOI: 10.1007/s00221-014-4088-5. View