Acellular Myocardial Scaffolds and Slices Fabrication, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2022 May 26

PMID 35618898

Authors

Bo Wang

Mickey Shah

Lakiesha N Williams

Amy L de Jongh Curry

Yi Hong

Ge Zhang

Jun Liao

Affiliations

Soon will be listed here.

Abstract

Cardiac tissue engineering/regeneration using decellularized myocardium has attracted great research attention due to its potential benefit to myocardial infarction (MI) treatment. Here, we described an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae, myocardial slices as a biomimetic cell culture and delivery platform, and a multi-stimulation bioreactor that is able to provide coordinated mechanical and electrical stimulations for facilitating cardiac construct development.

Citing Articles

Evaluation of Different Decellularization Protocols for Obtaining and Characterizing Canine Cardiac Extracellular Matrix.

da Silva I, Miglino M, de Souza S, Buchaim D, Buchaim R Biomedicines. 2024; 12(6).

PMID: 38927398 PMC: 11200447. DOI: 10.3390/biomedicines12061190.

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