A TEMPOL and Rapamycin Loaded Nanofiber-covered Stent Favors Endothelialization and Mitigates Neointimal Hyperplasia and Local Inflammation

Overview

Journal Bioact Mater

Specialty Biomedical Engineering

Date 2022 May 23

PMID 35600979

Authors

Rui Wang

Jian Lu

Jiasheng Yin

Han Chen

Hongmei Liu

Fei Xu

Tongtong Zang

Rende Xu

Chenguang Li

Yizhe Wu

Qilin Wu

Xiang Fei

Meifang Zhu

Li Shen

Junbo Ge

Affiliations

Soon will be listed here.

Abstract

An increased level of reactive oxygen species (ROS) plays a major role in endothelial dysfunction and vascular smooth muscle cell (VSMC) proliferation during in-stent thrombosis and restenosis after coronary artery stenting. Herein, we report an electrospun core-shell nanofiber coloaded with 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL) and rapamycin (RAPA) that correspondingly serves as an ROS scavenger and VSMC inhibitor. This system has the potential to improve the biocompatibility of current drug-eluting stent (DES) coatings with the long-term and continuous release of TEMPOL and rapamycin. Moreover, the RAPA/TEMPOL-loaded membrane selectively inhibited the proliferation of VSMCs while sparing endothelial cells (ECs). This membrane demonstrated superior ROS-scavenging, anti-inflammatory and antithrombogenic effects in ECs. In addition, the membrane could maintain the contractile phenotype and mitigate platelet-derived growth factor BB (PDGF-BB)-induced proliferation of VSMCs. results further revealed that the RAPA/TEMPOL-loaded covered stents promoted rapid restoration of vascular endothelium compared with DES and persistently impeded inflammation and neointimal hyperplasia in porcine models.

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