Protein ROMP: Aqueous Graft-from Ring-Opening Metathesis Polymerization

Ring-opening metathesis polymerization (ROMP) was carried out from the surface of a protein under aqueous conditions. Grubbs' third generation catalyst was modified with PEGylated pyridyl groups to form a water-soluble species that showed high activity in aqueous buffered solutions at near-neutral pH. The modification of a protein with this catalyst to create a stable macroinitiator for ROMP is described. The protein macroinitiator was then used to polymerize water-soluble norbornenes, resulting in high molecular weight protein/polymer conjugates. Varying polymerization time and monomer concentration demonstrated the kinetics of molecular weight evolution and macroinitiator conversion of graft-from ROMP.