Isolation and Structural Characterization of Two Polysaccharides from Dracocephalum Moldavica and Their Anti-Complementary Activity

The two novel polysaccharides, DMP-1 and DMP-2, with molecular weights of 4.1553×10  kDa and 1.9764×10  kDa, respectively, were isolated from Dracocephalum moldavica. The structural characterization indicated that DMP-1 and DMP-2 shared a similar backbone consisting of →5)-Araf-(1→, Manp-(1→, Glcp-(1→, →2)-Manp-(1→, →6)-Glcp-(1→ and →3,6)-Galp-(1→ with a different molar ratios and triple-helix structures with α- and β-type glycosidic bonds. The anti-complementary activity evaluation showed that DMP-1 and DMP-2 had strong complement inhibition through the classical pathway (CP), alternative pathway (AP) and lectin pathway (LP). Mechanistic studies indicated that DMP-1 can block the activation cascade of the complement system by targeting C2, C3, C5, C9, Factor B and Factor P, and that DMP-2 inhibited complement activation by blocking C2, C3, C4, C5, C9 and Factor B.