Nicotinic Acetylcholine Receptor Subunits and Their Native Interactions with Insecticidal Peptide Toxins

Overview

Journal Elife

Specialty Biology

Date 2022 May 16

PMID 35575460

Authors

Dagmara Korona

Benedict Dirnberger

Carlo N G Giachello

Rayner M L Queiroz

Rebeka Popovic

Karin H Muller

David-Paul Minde

Michael J Deery

Glynnis Johnson

Lucy C Firth

Fergus G Earley

Steven Russell

Kathryn S Lilley

Affiliations

Soon will be listed here.

Abstract

nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that represent a target for insecticides. Peptide neurotoxins are known to block nAChRs by binding to their target subunits, however, a better understanding of this mechanism is needed for effective insecticide design. To facilitate the analysis of nAChRs we used a CRISPR/Cas9 strategy to generate null alleles for all ten subunit genes in a common genetic background. We studied interactions of nAChR subunits with peptide neurotoxins by larval injections and styrene maleic acid lipid particles (SMALPs) pull-down assays. For the null alleles, we determined the effects of α-Bungarotoxin (α-Btx) and ω-Hexatoxin-Hv1a (Hv1a) administration, identifying potential receptor subunits implicated in the binding of these toxins. We employed pull-down assays to confirm α-Btx interactions with the α5 (D5), Dα6, D7 subunits. Finally, we report the localisation of fluorescent tagged endogenous Dα6 during CNS development. Taken together, this study elucidates native nAChR subunit interactions with insecticidal peptide toxins and provides a resource for the in vivo analysis of insect nAChRs.

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