Microfluidics-Based Time-Resolved Fluorescence Immunoassay for the On-Site Detection of Aflatoxins B1 Zearalenone and Deoxynivalenol in Cereals

Overview

Journal Foods

Specialty Biotechnology

Date 2022 May 14

PMID 35564042

Authors

Xu Wang

Disha Lu

Qingfeng Huang

Jinyi Yang

Affiliations

Soon will be listed here.

Abstract

The primary pollutants in cereal products are aflatoxins B1 (AFB1), zearalenone (ZEN), and deoxynivalenol (DON). In this study, anti-AFB1 MAb (4C9), anti-ZEN MAb (2A3), and anti-DON MAb (1F10) were developed and used in time-resolved fluorescence immunoassay microfluidics to determine AFB1, ZEN, and DON in agricultural products. The linear range for AFB1, ZEN, and DON were 0.05~2.2 μg/kg, 1.45~375.75 μg/kg, and 11.1~124.2 μg/kg, respectively. In maize, the recoveries of AFB1/ZEN/DON were 92~101%, 102~105%, and 103~108%, respectively. High-performance liquid chromatography and the proposed approach had a good correlation. Time-resolved fluorescence immunoassay microfluidics is a highly efficient and sensitive field detection method for fungal toxins in agricultural products.

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References

Camenzuli L, van Dam R, de Rijk T, Andriessen R, Van Schelt J, van der Fels-Klerx H . Tolerance and Excretion of the Mycotoxins Aflatoxin B₁, Zearalenone, Deoxynivalenol, and Ochratoxin A by Alphitobius diaperinus and Hermetia illucens from Contaminated Substrates. Toxins (Basel). 2018; 10(2). PMC: 5848191. DOI: 10.3390/toxins10020091. View

Balazs A, Faisal Z, Csepregi R, Koszegi T, Kriszt B, Szabo I . In Vitro Evaluation of the Individual and Combined Cytotoxic and Estrogenic Effects of Zearalenone, Its Reduced Metabolites, Alternariol, and Genistein. Int J Mol Sci. 2021; 22(12). PMC: 8230625. DOI: 10.3390/ijms22126281. View

Hervas M, Lopez M, Escarpa A . Electrochemical microfluidic chips coupled to magnetic bead-based ELISA to control allowable levels of zearalenone in baby foods using simplified calibration. Analyst. 2009; 134(12):2405-11. DOI: 10.1039/b911839j. View

Machado J, Soares R, Chu V, Conde J . Multiplexed capillary microfluidic immunoassay with smartphone data acquisition for parallel mycotoxin detection. Biosens Bioelectron. 2017; 99:40-46. DOI: 10.1016/j.bios.2017.07.032. View

Tang X, Li P, Zhang Q, Zhang Z, Zhang W, Jiang J . Time-Resolved Fluorescence Immunochromatographic Assay Developed Using Two Idiotypic Nanobodies for Rapid, Quantitative, and Simultaneous Detection of Aflatoxin and Zearalenone in Maize and Its Products. Anal Chem. 2017; 89(21):11520-11528. DOI: 10.1021/acs.analchem.7b02794. View

Hussein H, Brasel J . Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxicology. 2001; 167(2):101-34. DOI: 10.1016/s0300-483x(01)00471-1. View

Soares R, Santos D, Chu V, Azevedo A, Aires-Barros M, Conde J . A point-of-use microfluidic device with integrated photodetector array for immunoassay multiplexing: Detection of a panel of mycotoxins in multiple samples. Biosens Bioelectron. 2016; 87:823-831. DOI: 10.1016/j.bios.2016.09.041. View

Liu Y, Lu Y, Wang L, Chang F, Yang L . Occurrence of deoxynivalenol in wheat, Hebei Province, China. Food Chem. 2015; 197 Pt B:1271-4. DOI: 10.1016/j.foodchem.2015.11.047. View

Xiulan S, Xiaolian Z, Jian T, Zhou J, Chu F . Preparation of gold-labeled antibody probe and its use in immunochromatography assay for detection of aflatoxin B1. Int J Food Microbiol. 2005; 99(2):185-94. DOI: 10.1016/j.ijfoodmicro.2004.07.021. View

10.

Rodriguez-Carrasco Y, Molto J, Manes J, Berrada H . Development of a GC-MS/MS strategy to determine 15 mycotoxins and metabolites in human urine. Talanta. 2014; 128:125-31. DOI: 10.1016/j.talanta.2014.04.072. View

11.

Kong W, Wei R, Logrieco A, Wei J, Wen J, Xiao X . Occurrence of toxigenic fungi and determination of mycotoxins by HPLC-FLD in functional foods and spices in China markets. Food Chem. 2013; 146:320-6. DOI: 10.1016/j.foodchem.2013.09.005. View

12.

Shao Y, Duan H, Zhou S, Ma T, Guo L, Huang X . Biotin-Streptavidin System-Mediated Ratiometric Multiplex Immunochromatographic Assay for Simultaneous and Accurate Quantification of Three Mycotoxins. J Agric Food Chem. 2019; 67(32):9022-9031. DOI: 10.1021/acs.jafc.9b03222. View

13.

Chen Y, Meng X, Zhu Y, Shen M, Lu Y, Cheng J . Rapid detection of four mycotoxins in corn using a microfluidics and microarray-based immunoassay system. Talanta. 2018; 186:299-305. DOI: 10.1016/j.talanta.2018.04.064. View

14.

Lee H, Ryu D . Worldwide Occurrence of Mycotoxins in Cereals and Cereal-Derived Food Products: Public Health Perspectives of Their Co-occurrence. J Agric Food Chem. 2016; 65(33):7034-7051. DOI: 10.1021/acs.jafc.6b04847. View

15.

SWEENEY M, Dobson A . Mycotoxin production by Aspergillus, Fusarium and Penicillium species. Int J Food Microbiol. 1998; 43(3):141-58. DOI: 10.1016/s0168-1605(98)00112-3. View

16.

Rice L, Ross P . Methods for Detection and Quantitation of Fumonisins in Corn, Cereal Products and Animal Excreta. J Food Prot. 2019; 57(6):536-540. DOI: 10.4315/0362-028X-57.6.536. View

17.

Wei Y, Zhao X, Li M, Yu J, Gurudeeban S, Hu Y . Detoxification of aflatoxins on prospective approach: effect on structural, mechanical, and optical properties under pressures. Interdiscip Sci. 2017; 10(2):311-319. DOI: 10.1007/s12539-017-0278-8. View

18.

Foubert A, Beloglazova N, De Saeger S . Comparative study of colloidal gold and quantum dots as labels for multiplex screening tests for multi-mycotoxin detection. Anal Chim Acta. 2017; 955:48-57. DOI: 10.1016/j.aca.2016.11.042. View

19.

Xu L, Zhang Z, Zhang Q, Zhang W, Yu L, Wang D . An On-Site Simultaneous Semi-quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-based Feed via Multicolor Immunochromatographic Assay. Toxins (Basel). 2018; 10(2). PMC: 5848188. DOI: 10.3390/toxins10020087. View

20.

Wu Q, Kuca K, Humpf H, Klimova B, Cramer B . Fate of deoxynivalenol and deoxynivalenol-3-glucoside during cereal-based thermal food processing: a review study. Mycotoxin Res. 2016; 33(1):79-91. DOI: 10.1007/s12550-016-0263-9. View