Comprehensive Analysis of Long Noncoding RNA Modified by MA Methylation in Oxidative and Glycolytic Skeletal Muscles

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 May 14

PMID 35562992

Authors

Shanshan Wang

Baohua Tan

Liyao Xiao

Xinming Zhao

Jiekang Zeng

Linjun Hong

Jie Yang

Gengyuan Cai

Enqin Zheng

Zhenfang Wu

Ting Gu

Affiliations

Soon will be listed here.

Abstract

N-methyladenosine (mA) is the most common modification in eukaryotic RNAs. Accumulating evidence shows mA methylation plays vital roles in various biological processes, including muscle and fat differentiation. However, there is a lack of research on lncRNAs' mA modification in regulating pig muscle-fiber-type conversion. In this study, we identified novel and differentially expressed lncRNAs in oxidative and glycolytic skeletal muscles through RNA-seq, and further reported the mA-methylation patterns of lncRNAs via MeRIP-seq. We found that most lncRNAs have one mA peak, and the mA peaks were preferentially enriched in the last exon of the lncRNAs. Interestingly, we found that lncRNAs' mA levels were positively correlated with their expression homeostasis and levels. Furthermore, we performed conjoint analysis of MeRIP-seq and RNA-seq data and obtained 305 differentially expressed and differentially mA-modified lncRNAs (dme-lncRNAs). Through QTL enrichment analysis of dme-lncRNAs and PPI analysis for their cis-genes, we finally identified seven key mA-modified lncRNAs that may play a potential role in muscle-fiber-type conversion. Notably, inhibition of one of the key lncRNAs, , delayed satellite cell differentiation and stimulated fast-to-slow muscle-fiber conversion. Our study comprehensively analyzed mA modifications on lncRNAs in oxidative and glycolytic skeletal muscles and provided new targets for the study of pig muscle-fiber-type conversion.

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