Development of Online-storage Inner-recycling Counter-current Chromatography for the Preparative Separation of Complex Components of Alkylphenols from Sarcotesta of L

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 13

PMID 35548654

Authors

Daijie Wang

Xiangyun Song

Huijiao Yan

Mengmeng Guo

Ruiming Fu

Hailong Jiang

Heng Zhu

Xiao Wang

Affiliations

Soon will be listed here.

Abstract

High-speed counter-current chromatography (HSCCC) is becoming an effective and non-absorptive separation method from natural products. Due to the insufficient separation efficiency, it is challenging to separate complex components, especially for compounds with similar values. In this study, a novel and effective online-storage inner-recycling CCC method was used to separate alkylphenols from the sarcotesta of L. A two-phase solvent system of -heptane/ethyl acetate/methanol/acetic acid (5 : 4 : 1 : 1, v/v) was used for HSCCC separation of 500 mg crude extracts. After the inner-recycling of two fractions coupled with pre-HPLC, five main ginkgolic acids (C13:0, C15:1, C17:2, C15:1, C17:1) coupled with bilobol (C15:1) and a mixture were obtained from a non-stop separation using a storage loop and two six-way valves. This novel method was also evaluated and predicted by formula derivation. This method could be an effective, rapid, and simple approach to separate alkylphenols from the sarcotesta of .

Citing Articles

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Xu T, Wu X RSC Adv. 2022; 10(43):25780-25785.

PMID: 35518602 PMC: 9055302. DOI: 10.1039/d0ra04307a.

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