Efficacy of Ginkgolic Acids Against Cryptosporidium Andersoni in Cell Culture

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2011 May 11

PMID 21556686

Citations 6

Authors

Liang Wu

Xu-Gan Jiang

Yu-Juan Shen

Zhao-Xi Lu

Guo-Hua Tu

Xing-Li Fu

Sheng-Xia Chen

Jian-Ping Cao

Affiliations

Soon will be listed here.

Abstract

Cryptosporidium is a worldwide waterborne parasite and the treatment is a severe problem in immunocompromised patients. In this study, we used the in vitro culture system to evaluate the anti-Cryptosporidium activity of ginkgolic acids (GAs), nitazoxanide (NTZ), garlicin (GAR), and artemether (ART). The growth of Cryptosporidium andersoni in HCT-8 cells was determined by real-time PCR assay. When exposed to 5.00 μg/ml GAs or 10.00 μg/ml NTZ for 48 h, the number of C. andersoni in cultures was on a very low lever, but the number of parasites did not significantly decrease when exposed to GAR and ART. Our results indicate that GAs could be a potential drug for the treatment of cryptosporidiosis.

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References

You X, Schinazi R, Arrowood M, Lejkowski M, Juodawlkis A, Mead J . In-vitro activities of paromomycin and lasalocid evaluated in combination against Cryptosporidium parvum. J Antimicrob Chemother. 1998; 41(2):293-6. DOI: 10.1093/jac/41.2.293. View

Yang X, Chen J, Qian Z, Guo T . [Study on the antibacterial activity of ginkgolic acids]. Zhong Yao Cai. 2002; 25(9):651-3. View

Zhao Q, Zhang M, Hong L, Zhou K, Lin Y . Evaluation of drug effects on Toxoplasma gondii nuclear and plastid DNA replication using real-time PCR. Parasitol Res. 2010; 106(5):1257-62. DOI: 10.1007/s00436-010-1792-3. View

Anceno A, Katayama H, Houpt E, Chavalitshewinkoon-Petmitr P, Chuluun B, Shipin O . IMS-free DNA extraction for the PCR-based quantification of Cryptosporidium parvum and Giardia lamblia in surface and waste water. Int J Environ Health Res. 2007; 17(4):297-310. DOI: 10.1080/09603120701372573. View

Chen X, Keithly J, Paya C, LaRusso N . Cryptosporidiosis. N Engl J Med. 2002; 346(22):1723-31. DOI: 10.1056/NEJMra013170. View

Theodos C, Griffiths J, DOnfro J, Fairfield A, Tzipori S . Efficacy of nitazoxanide against Cryptosporidium parvum in cell culture and in animal models. Antimicrob Agents Chemother. 1998; 42(8):1959-65. PMC: 105716. DOI: 10.1128/AAC.42.8.1959. View

Chen S, Wu L, Jiang X, Feng Y, Cao J . Anti-Toxoplasma gondii activity of GAS in vitro. J Ethnopharmacol. 2008; 118(3):503-7. DOI: 10.1016/j.jep.2008.05.023. View

Wu L, Chen S, Jiang X, Shen Y, Lu Z, Tu G . Effect of select medium supplements on in vitro development of Cryptosporidium andersoni in HCT-8 cells. Parasitol Res. 2009; 105(5):1419-24. DOI: 10.1007/s00436-009-1576-9. View

Lindsay D, Upton S, Owens D, Morgan U, Mead J, Blagburn B . Cryptosporidium andersoni n. sp. (Apicomplexa: Cryptosporiidae) from cattle, Bos taurus. J Eukaryot Microbiol. 2000; 47(1):91-5. DOI: 10.1111/j.1550-7408.2000.tb00016.x. View

10.

Guyot K, Follet-Dumoulin A, Lelievre E, Sarfati C, Rabodonirina M, Nevez G . Molecular characterization of Cryptosporidium isolates obtained from humans in France. J Clin Microbiol. 2001; 39(10):3472-80. PMC: 88374. DOI: 10.1128/JCM.39.10.3472-3480.2001. View

11.

Zhu G, Marchewka M, Keithly J . Cryptosporidium parvum possesses a short-type replication protein A large subunit that differs from its host. FEMS Microbiol Lett. 1999; 176(2):367-72. DOI: 10.1111/j.1574-6968.1999.tb13685.x. View

12.

Arrowood M, Sterling C . Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J Parasitol. 1987; 73(2):314-9. View

13.

Xiao L, Singh A, Limor J, Graczyk T, Gradus S, Lal A . Molecular characterization of cryptosporidium oocysts in samples of raw surface water and wastewater. Appl Environ Microbiol. 2001; 67(3):1097-101. PMC: 92700. DOI: 10.1128/AEM.67.3.1097-1101.2001. View

14.

Cai X, Woods K, Upton S, Zhu G . Application of quantitative real-time reverse transcription-PCR in assessing drug efficacy against the intracellular pathogen Cryptosporidium parvum in vitro. Antimicrob Agents Chemother. 2005; 49(11):4437-42. PMC: 1280145. DOI: 10.1128/AAC.49.11.4437-4442.2005. View

15.

Di Giovanni G, LeChevallier M . Quantitative-PCR assessment of Cryptosporidium parvum cell culture infection. Appl Environ Microbiol. 2005; 71(3):1495-500. PMC: 1065146. DOI: 10.1128/AEM.71.3.1495-1500.2005. View

16.

Amadi B, Mwiya M, Sianongo S, Payne L, Watuka A, Katubulushi M . High dose prolonged treatment with nitazoxanide is not effective for cryptosporidiosis in HIV positive Zambian children: a randomised controlled trial. BMC Infect Dis. 2009; 9:195. PMC: 2794874. DOI: 10.1186/1471-2334-9-195. View

17.

Liu H, Cao J, Shen Y, Chen Y, Li X, Lu W . [Isolation and identification of an isolate of cow-origin Cryptosporidium sp]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2007; 25(2):81-6. View

18.

Rossignol J . Cryptosporidium and Giardia: treatment options and prospects for new drugs. Exp Parasitol. 2009; 124(1):45-53. DOI: 10.1016/j.exppara.2009.07.005. View

19.

Zardi E, Picardi A, Afeltra A . Treatment of cryptosporidiosis in immunocompromised hosts. Chemotherapy. 2005; 51(4):193-6. DOI: 10.1159/000086920. View

20.

Mosmann T . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63. DOI: 10.1016/0022-1759(83)90303-4. View