Bioceramic Akermanite Enhanced Vascularization and Osteogenic Differentiation of Human Induced Pluripotent Stem Cells in 3D Scaffolds and

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 9

PMID 35530104

Authors

Xixi Dong

Haiyan Li

Lingling E

Junkai Cao

Bin Guo

Affiliations

Soon will be listed here.

Abstract

A growing number of studies suggest that the modulation of cell differentiation by biomaterials is critical for tissue engineering. In previous work, we demonstrated that human induced pluripotent stem cells (iPSCs) are remarkably promising seed cells for bone tissue engineering. In addition, we found that the ionic products of akermanite (Aker) are potential inducers of osteogenic differentiation of iPSCs. Furthermore, composite scaffolds containing polymer and bioceramics have more interesting properties compared to pure bioceramic scaffolds for bone tissue engineering. The characteristic of model biomaterials in bone tissue engineering is their ability to control the osteogenic differentiation of stem cells and simultaneously induce the angiogenesis of endothelia cells. Thus, this study aimed at investigating the effects of poly(lactic--glycolic acid)/Aker (PLGA-Aker) composite scaffolds on angiogenic and osteogenic differentiation of human iPSCs in order to optimize the scaffold compositions. The results from Alizarin Red S staining, qRT-PCR analysis of osteogenic genes (BMP2, RUNX2, ALP, COL1 and OCN) and angiogenic genes (VEGF and CD31) demonstrated that PLGA/Aker composite scaffolds containing 10% Aker exhibited the highest stimulatory effects on the osteogenic and angiogenic differentiation of human iPSCs among all scaffolds. After the scaffolds were implanted in nu/nu mice subcutaneous pockets and calvarial defects, H&E staining, BSP immunostaining, qRT-PCR analysis and micro-CT analysis (BMD, BV/TV) indicated that PLGA + 10% Aker scaffolds enhanced the vascularization and osteogenic differentiation of human iPSCs and stimulated the repair of bone defects. Taken together, our work indicated that combining scaffolds containing silicate bioceramic Aker and human iPSCs is a promising approach for the enhancement of bone regeneration.

Citing Articles

Enhanced Vascularity in Gelatin Scaffolds via Copper-Doped Magnesium-Calcium Silicates Incorporation: and Insights.

Salahinejad E, Muralidharan A, Sayahpour F, Kianpour M, Akbarian M, Vashaee D Ceram Int. 2024; 50(20 Pt B):39889-39897.

PMID: 39618431 PMC: 11606534. DOI: 10.1016/j.ceramint.2024.07.369.

Enhancing Mechanical and Biological Properties of Zinc Phosphate Dental Cement with Akermanite and Hardystonite Nanoparticles: A Synthesis and Characterization Study.

Eslami H, Ansari M, Khademi R, Zare-Zardini H Int J Dent. 2024; 2024:4916315.

PMID: 39238600 PMC: 11377109. DOI: 10.1155/2024/4916315.

Stem Cells in Bone Tissue Engineering: Progress, Promises and Challenges.

Augustine R, Gezek M, Nikolopoulos V, Buck P, Bostanci N, Camci-Unal G Stem Cell Rev Rep. 2024; 20(7):1692-1731.

PMID: 39028416 DOI: 10.1007/s12015-024-10738-y.

Mesenchymal stem cells in craniofacial reconstruction: a comprehensive review.

Zheng Z, Liu H, Liu S, Luo E, Liu X Front Mol Biosci. 2024; 11:1362338.

PMID: 38690295 PMC: 11058977. DOI: 10.3389/fmolb.2024.1362338.

Magnesium-containing bioceramics stimulate exosomal miR-196a-5p secretion to promote senescent osteogenesis through targeting /MAPK signaling axis.

Qi L, Fang X, Yan J, Pan C, Ge W, Wang J Bioact Mater. 2023; 33:14-29.

PMID: 38024235 PMC: 10661166. DOI: 10.1016/j.bioactmat.2023.10.024.

References

Sun H, Wu C, Dai K, Chang J, Tang T . Proliferation and osteoblastic differentiation of human bone marrow-derived stromal cells on akermanite-bioactive ceramics. Biomaterials. 2006; 27(33):5651-7. DOI: 10.1016/j.biomaterials.2006.07.027. View

Zhai W, Lu H, Wu C, Chen L, Lin X, Naoki K . Stimulatory effects of the ionic products from Ca-Mg-Si bioceramics on both osteogenesis and angiogenesis in vitro. Acta Biomater. 2013; 9(8):8004-14. DOI: 10.1016/j.actbio.2013.04.024. View

Li H, Xue K, Kong N, Liu K, Chang J . Silicate bioceramics enhanced vascularization and osteogenesis through stimulating interactions between endothelia cells and bone marrow stromal cells. Biomaterials. 2014; 35(12):3803-18. DOI: 10.1016/j.biomaterials.2014.01.039. View

Chen J, Crawford R, Chen C, Xiao Y . The key regulatory roles of the PI3K/Akt signaling pathway in the functionalities of mesenchymal stem cells and applications in tissue regeneration. Tissue Eng Part B Rev. 2013; 19(6):516-28. DOI: 10.1089/ten.TEB.2012.0672. View

Wu Y, Xia L, Zhou Y, Xu Y, Jiang X . Icariin induces osteogenic differentiation of bone mesenchymal stem cells in a MAPK-dependent manner. Cell Prolif. 2015; 48(3):375-84. PMC: 6496185. DOI: 10.1111/cpr.12185. View

Wang C, Lin K, Chang J, Sun J . Osteogenesis and angiogenesis induced by porous β-CaSiO(3)/PDLGA composite scaffold via activation of AMPK/ERK1/2 and PI3K/Akt pathways. Biomaterials. 2012; 34(1):64-77. DOI: 10.1016/j.biomaterials.2012.09.021. View

Day R . Bioactive glass stimulates the secretion of angiogenic growth factors and angiogenesis in vitro. Tissue Eng. 2005; 11(5-6):768-77. DOI: 10.1089/ten.2005.11.768. View

Peter S, Miller M, Yasko A, Yaszemski M, Mikos A . Polymer concepts in tissue engineering. J Biomed Mater Res. 1998; 43(4):422-7. DOI: 10.1002/(sici)1097-4636(199824)43:4<422::aid-jbm9>3.0.co;2-1. View

Li H, Chang J . Preparation and characterization of bioactive and biodegradable wollastonite/poly(D,L-lactic acid) composite scaffolds. J Mater Sci Mater Med. 2004; 15(10):1089-95. DOI: 10.1023/B:JMSM.0000046390.09540.c2. View

10.

Zhang M, Wu C, Lin K, Fan W, Chen L, Xiao Y . Biological responses of human bone marrow mesenchymal stem cells to Sr-M-Si (M = Zn, Mg) silicate bioceramics. J Biomed Mater Res A. 2012; 100(11):2979-90. DOI: 10.1002/jbm.a.34246. View

11.

Lin K, Xia L, Li H, Jiang X, Pan H, Xu Y . Enhanced osteoporotic bone regeneration by strontium-substituted calcium silicate bioactive ceramics. Biomaterials. 2013; 34(38):10028-42. DOI: 10.1016/j.biomaterials.2013.09.056. View

12.

Dong X, Li H, Zhou Y, Ou L, Cao J, Chang J . The stimulation of osteogenic differentiation of embryoid bodies from human induced pluripotent stem cells by akermanite bioceramics. J Mater Chem B. 2020; 4(13):2369-2376. DOI: 10.1039/c6tb00398b. View

13.

Huang X, Zhang X, Wang X, Wang C, Tang B . Microenvironment of alginate-based microcapsules for cell culture and tissue engineering. J Biosci Bioeng. 2012; 114(1):1-8. DOI: 10.1016/j.jbiosc.2012.02.024. View

14.

Xu S, Lin K, Wang Z, Chang J, Wang L, Lu J . Reconstruction of calvarial defect of rabbits using porous calcium silicate bioactive ceramics. Biomaterials. 2008; 29(17):2588-96. DOI: 10.1016/j.biomaterials.2008.03.013. View

15.

Kaigler D, Krebsbach P, West E, Horger K, Huang Y, Mooney D . Endothelial cell modulation of bone marrow stromal cell osteogenic potential. FASEB J. 2005; 19(6):665-7. DOI: 10.1096/fj.04-2529fje. View

16.

Bezenah J, Rioja A, Juliar B, Friend N, Putnam A . Assessing the ability of human endothelial cells derived from induced-pluripotent stem cells to form functional microvasculature in vivo. Biotechnol Bioeng. 2018; 116(2):415-426. PMC: 6322937. DOI: 10.1002/bit.26860. View

17.

Huang Y, Jin X, Zhang X, Sun H, Tu J, Tang T . In vitro and in vivo evaluation of akermanite bioceramics for bone regeneration. Biomaterials. 2009; 30(28):5041-8. DOI: 10.1016/j.biomaterials.2009.05.077. View

18.

Xie J, Peng C, Zhao Q, Wang X, Yuan H, Yang L . Osteogenic differentiation and bone regeneration of iPSC-MSCs supported by a biomimetic nanofibrous scaffold. Acta Biomater. 2015; 29:365-379. DOI: 10.1016/j.actbio.2015.10.007. View

19.

Kim J, Hou L, Yang G, Mezak N, Wanjare M, Joubert L . Microfibrous Scaffolds Enhance Endothelial Differentiation and Organization of Induced Pluripotent Stem Cells. Cell Mol Bioeng. 2017; 10(5):417-432. PMC: 5602598. DOI: 10.1007/s12195-017-0502-y. View

20.

Son J, Appleford M, Ong J, Wenke J, Kim J, Choi S . Porous hydroxyapatite scaffold with three-dimensional localized drug delivery system using biodegradable microspheres. J Control Release. 2011; 153(2):133-40. DOI: 10.1016/j.jconrel.2011.03.010. View