SAturated Transposon Analysis in Yeast (SATAY) for Deep Functional Mapping of Yeast Genomes

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2022 May 6

PMID 35524127

Authors

Agnes H Michel

Benoit Kornmann

Affiliations

Soon will be listed here.

Abstract

Genome-wide transposon mutagenesis followed by deep sequencing allows the genome-wide mapping of growth-affecting loci in a straightforward and time-efficient way.SAturated Transposon Analysis in Yeast (SATAY) takes advantage of a modified maize transposon that is highly mobilizable in S. cerevisiae. SATAY allows not only the genome-wide mapping of genes required for growth in select conditions (such as genetic interactions or drug sensitivity/resistance), but also of protein sub-domains, as well as the creation of gain- and separation-of-function alleles. From strain preparation to the mapping of sequencing reads, we detail all the steps for the making and analysis of SATAY libraries in any S. cerevisiae lab, requiring only ordinary equipment and access to a Next-Gen sequencing platform.

Citing Articles

Saturated Transposon Analysis in Yeast as a one-step method to quantify the fitness effects of gene disruptions on a genome-wide scale.

Kingma E, Dolsma F, Inigo de la Cruz L, Laan L PLoS One. 2025; 20(2):e0312437.

PMID: 39913404 PMC: 11801604. DOI: 10.1371/journal.pone.0312437.

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