Co-localized Line-field Confocal Optical Coherence Tomography and Confocal Raman Microspectroscopy for Three-dimensional High-resolution Morphological and Molecular Characterization of Skin Tissues

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2022 May 6

PMID 35519243

Authors

Lena Waszczuk

Jonas Ogien

Jean-Luc Perrot

Arnaud Dubois

Affiliations

Soon will be listed here.

Abstract

Line-field confocal optical coherence tomography (LC-OCT) is an optical modality that provides three-dimensional (3D) images of the skin at cellular resolution. Confocal Raman microspectroscopy (CRM) is a label-free optical technique that can provide point measurement of the molecular content of the skin. This work presents a method to co-localize LC-OCT and CRM acquisitions for morpho-molecular analysis of skin tissues at cellular level. The co-localization method allows acquisition of Raman spectra at specific locations in a sample identified from a 3D LC-OCT image, with an accuracy of ± 20 µm. The method was applied to the characterization of tattooed skin biopsies with adverse tattoo reactions. LC-OCT images allowed to target specific regions in the biopsies where the presence of tattoo ink was revealed by detection of the Raman signature of ink pigments. Micrometer-sized foreign bodies of various materials as well as inflammatory cells were also identified within the biopsies. From these results, we demonstrate the value of the LC-OCT-CRM co-localization method and its potential for future analysis of suspicious skin lesions.

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