Messenger RNA Stability in Saccharomyces Cerevisiae: the Influence of Translation and Poly(A) Tail Length

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Mar 25

PMID 3550698

Citations 13

Authors

T C Santiago

A J Bettany

I J Purvis

A J Brown

Affiliations

Soon will be listed here.

Abstract

A comparison between the half-lives of 10 specific yeast mRNAs and their distribution within polysomes (fractionated on sucrose density gradients) was used to test the relationship between mRNA translation and degradation in the eukaryote Saccharomyces cerevisiae. Although the mRNAs vary in their distribution across the same polysome gradients, there is no obvious correlation between the stability of an mRNA and the number of ribosomes it carries in vivo. This suggests that ribosomal protection against nucleolytic attack is not a major factor in determining the stability of an mRNA in yeast. The relative lengths of the poly(A) tails of 9 yeast mRNAs were analysed using thermal elution from poly(U)-Sepharose. No dramatic differences in poly(A) tail length were observed amongst the mRNAs which could account for their wide ranging half-lives. Minor differences were consistent with shortening of the poly(A) tail as an mRNA ages.

Citing Articles

CEP63 upregulates YAP1 to promote colorectal cancer progression through stabilizing RNA binding protein FXR1.

Ling H, Cao C, Han K, Lv Y, Ma X, Cao J Oncogene. 2022; 41(39):4433-4445.

PMID: 35989368 DOI: 10.1038/s41388-022-02439-y.

Messenger RNAs of Yeast Virus-Like Elements Contain Non-templated 5' Poly(A) Leaders, and Their Expression Is Independent of eIF4E and Pab1.

Vopalensky V, Sykora M, Masek T, Pospisek M Front Microbiol. 2019; 10:2366.

PMID: 31736885 PMC: 6831550. DOI: 10.3389/fmicb.2019.02366.

Comprehensive analysis of nucleocytoplasmic dynamics of mRNA in Drosophila cells.

Chen T, van Steensel B PLoS Genet. 2017; 13(8):e1006929.

PMID: 28771467 PMC: 5557608. DOI: 10.1371/journal.pgen.1006929.

Mechanisms and control of mRNA turnover in Saccharomyces cerevisiae.

Caponigro G, Parker R Microbiol Rev. 1996; 60(1):233-49.

PMID: 8852902 PMC: 239426. DOI: 10.1128/mr.60.1.233-249.1996.

Glucose-dependent turnover of the mRNAs encoding succinate dehydrogenase peptides in Saccharomyces cerevisiae: sequence elements in the 5' untranslated region of the Ip mRNA play a dominant role.

Cereghino G, Atencio D, Saghbini M, Beiner J, Scheffler I Mol Biol Cell. 1995; 6(9):1125-43.

PMID: 8534911 PMC: 301272. DOI: 10.1091/mbc.6.9.1125.

References

Lindquist S . Regulation of protein synthesis during heat shock. Nature. 1981; 293(5830):311-4. DOI: 10.1038/293311a0. View

Brown A, Hardman N . The effect of age on the properties of poly(A)-containing messenger RNA in Physarum polycephalum. J Gen Microbiol. 1981; 122(1):143-50. DOI: 10.1099/00221287-122-1-143. View

Gorski J, Fiori M, MACH B . A new nonsense mutation as the molecular basis for beta thalassaemia. J Mol Biol. 1982; 154(3):537-40. DOI: 10.1016/s0022-2836(82)80012-0. View

Graham M, Tal M, Schlessinger D . lac Transcription in Escherichia coli cells treated with chloramphenicol. J Bacteriol. 1982; 151(1):251-61. PMC: 220234. DOI: 10.1128/jb.151.1.251-261.1982. View

LITTAUER U, Soreq H . The regulatory function of poly(A) and adjacent 3' sequences in translated RNA. Prog Nucleic Acid Res Mol Biol. 1982; 27:53-83. DOI: 10.1016/s0079-6603(08)60597-8. View

Burke R, Najarian R . The isolation, characterization, and sequence of the pyruvate kinase gene of Saccharomyces cerevisiae. J Biol Chem. 1983; 258(4):2193-201. View

Losson R, Fuchs R, Lacroute F . In vivo transcription of a eukaryotic regulatory gene. EMBO J. 1983; 2(12):2179-84. PMC: 555431. DOI: 10.1002/j.1460-2075.1983.tb01720.x. View

Zaret K, Sherman F . Mutationally altered 3' ends of yeast CYC1 mRNA affect transcript stability and translational efficiency. J Mol Biol. 1984; 177(1):107-35. DOI: 10.1016/0022-2836(84)90060-3. View

Krowczynska A, Yenofsky R, Brawerman G . Regulation of messenger RNA stability in mouse erythroleukemia cells. J Mol Biol. 1985; 181(2):231-9. DOI: 10.1016/0022-2836(85)90087-7. View

10.

Rabbitts P, Forster A, Stinson M, Rabbitts T . Truncation of exon 1 from the c-myc gene results in prolonged c-myc mRNa stability. EMBO J. 1985; 4(13B):3727-33. PMC: 554724. DOI: 10.1002/j.1460-2075.1985.tb04141.x. View

11.

Wong H, Chang S . Identification of a positive retroregulator that stabilizes mRNAs in bacteria. Proc Natl Acad Sci U S A. 1986; 83(10):3233-7. PMC: 323487. DOI: 10.1073/pnas.83.10.3233. View

12.

Belasco J, Nilsson G, von Gabain A, Cohen S . The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments. Cell. 1986; 46(2):245-51. DOI: 10.1016/0092-8674(86)90741-5. View

13.

Shaw G, Kamen R . A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell. 1986; 46(5):659-67. DOI: 10.1016/0092-8674(86)90341-7. View

14.

Baim S, Pietras D, Eustice D, Sherman F . A mutation allowing an mRNA secondary structure diminishes translation of Saccharomyces cerevisiae iso-1-cytochrome c. Mol Cell Biol. 1985; 5(8):1839-46. PMC: 366899. DOI: 10.1128/mcb.5.8.1839-1846.1985. View

15.

Santiago T, Purvis I, Bettany A, Brown A . The relationship between mRNA stability and length in Saccharomyces cerevisiae. Nucleic Acids Res. 1986; 14(21):8347-60. PMC: 311863. DOI: 10.1093/nar/14.21.8347. View

16.

Hutchison H, Hartwell L . Macromolecule synthesis in yeast spheroplasts. J Bacteriol. 1967; 94(5):1697-705. PMC: 276880. DOI: 10.1128/jb.94.5.1697-1705.1967. View

17.

Hamilton M, RUTH M . The dissociation of rat liver ribosomes by ethylenediaminetetraacetic acid; molecular weights, chemical composition, and buoyant densities of the subunits. Biochemistry. 1969; 8(3):851-6. DOI: 10.1021/bi00831a013. View

18.

Morse D, Yanofsky C . Polarity and the degradation of mRNA. Nature. 1969; 224(5217):329-31. DOI: 10.1038/224329a0. View

19.

Morse D . Polarity induced by chloramphenicol and relief by suA. J Mol Biol. 1971; 55(1):113-8. DOI: 10.1016/0022-2836(71)90285-3. View

20.

Hiraga S, Yanofsky C . Hyper-labile messenger RNA in polar mutants of the tryptophan operon of Escherichia coli. J Mol Biol. 1972; 72(1):103-10. DOI: 10.1016/0022-2836(72)90072-1. View