Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-like Cells

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2022 Apr 29

PMID 35486246

Authors

Federico Pecori

Hisatoshi Hanamatsu

Jun-Ichi Furukawa

Shoko Nishihara

Affiliations

Soon will be listed here.

Abstract

Glycosylation is one of the most abundant posttranslational modifications and is involved in a wide range of cellular processes. Glycome diversity in mammals is generated by the action of over 200 distinct glycosyltransferases and related enzymes. Nevertheless, glycosylation dynamics are tightly coordinated to allow proper organismal development. Here, using mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) as model systems, we describe a robust protocol that allows comprehensive and comparative structural analysis of the glycome.

References

Apweiler R, Hermjakob H, Sharon N . On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database. Biochim Biophys Acta. 1999; 1473(1):4-8. DOI: 10.1016/s0304-4165(99)00165-8. View

Pecori F, Yokota I, Hanamatsu H, Miura T, Ogura C, Ota H . A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition. Sci Rep. 2021; 11(1):1276. PMC: 7809059. DOI: 10.1038/s41598-020-79666-4. View

Nishihara S . Glycans in stem cell regulation: from Drosophila tissue stem cells to mammalian pluripotent stem cells. FEBS Lett. 2018; 592(23):3773-3790. DOI: 10.1002/1873-3468.13167. View

Varki A . Biological roles of glycans. Glycobiology. 2016; 27(1):3-49. PMC: 5884436. DOI: 10.1093/glycob/cww086. View

Hirayama H, Seino J, Kitajima T, Jigami Y, Suzuki T . Free oligosaccharides to monitor glycoprotein endoplasmic reticulum-associated degradation in Saccharomyces cerevisiae. J Biol Chem. 2010; 285(16):12390-404. PMC: 2852977. DOI: 10.1074/jbc.M109.082081. View

Bennett E, Mandel U, Clausen H, Gerken T, Fritz T, Tabak L . Control of mucin-type O-glycosylation: a classification of the polypeptide GalNAc-transferase gene family. Glycobiology. 2011; 22(6):736-56. PMC: 3409716. DOI: 10.1093/glycob/cwr182. View

Uematsu R, Furukawa J, Nakagawa H, Shinohara Y, Deguchi K, Monde K . High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis. Mol Cell Proteomics. 2005; 4(12):1977-89. DOI: 10.1074/mcp.M500203-MCP200. View

Furukawa J, Fujitani N, Araki K, Takegawa Y, Kodama K, Shinohara Y . A versatile method for analysis of serine/threonine posttranslational modifications by β-elimination in the presence of pyrazolone analogues. Anal Chem. 2011; 83(23):9060-7. DOI: 10.1021/ac2019848. View

Takegawa Y, Araki K, Fujitani N, Furukawa J, Sugiyama H, Sakai H . Simultaneous analysis of heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan disaccharides by glycoblotting-assisted sample preparation followed by single-step zwitter-ionic-hydrophilic interaction chromatography. Anal Chem. 2011; 83(24):9443-9. DOI: 10.1021/ac2021079. View

10.

Hanamatsu H, Nishikaze T, Miura N, Piao J, Okada K, Sekiya S . Sialic Acid Linkage Specific Derivatization of Glycosphingolipid Glycans by Ring-Opening Aminolysis of Lactones. Anal Chem. 2018; 90(22):13193-13199. DOI: 10.1021/acs.analchem.8b02775. View