Injectable Multifunctional CMC/HA-DA Hydrogel for Repairing Skin Injury

Overview

Journal Mater Today Bio

Specialty Biomedical Engineering

Date 2022 Apr 26

PMID 35469255

Authors

Longlong Cui

Jiankang Li

Shuaimeng Guan

Kaixiang Zhang

Kun Zhang

Jingan Li

Affiliations

Soon will be listed here.

Abstract

Injectable Hydrogels with adhesive, antioxidant and hemostatic properties are highly desired for promoting skin injury repair. In this study, we prepared a multi-functional carboxymethyl chitosan/hyaluronic acid-dopamine (CMC/HA-DA) hydrogel, which can be crosslinked by horseradish peroxidase and hydrogen peroxide. The antioxidation, gelation time, degradability, rheology and antihemorrhagic properties of hydrogels can be finely tuned by varying composition ratio. The cytocompatibility test and hemolysis test confirmed that the designed hydrogel holds good biocompatibility. More importantly, the repair effect of the hydrogel on full-thickness skin injury model in mice was studied. The results of wound healing, collagen deposition, immunohistochemistry and immunofluorescence showed that CMC/HA-DA hydrogel could significantly promote angiogenesis and cell proliferation at the injured site. Notably, the inflammatory response can also be regulated to promote the repair of full-thickness skin defect in mice. Results indicate that this injectable CMC/HA-DA hydrogel holds high application prospect for promising wound healing.

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References

Yao M, Gao F, Xu R, Zhang J, Chen Y, Guan F . A dual-enzymatically cross-linked injectable gelatin hydrogel loaded with BMSC improves neurological function recovery of traumatic brain injury in rats. Biomater Sci. 2019; 7(10):4088-4098. DOI: 10.1039/c9bm00749k. View

Li H, Cheng F, Wei X, Yi X, Tang S, Wang Z . Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing. Mater Sci Eng C Mater Biol Appl. 2020; 118:111324. DOI: 10.1016/j.msec.2020.111324. View

Hou B, Qi M, Sun J, Ai M, Ma X, Cai W . Preparation, characterization and wound healing effect of vaccarin-chitosan nanoparticles. Int J Biol Macromol. 2020; 165(Pt B):3169-3179. DOI: 10.1016/j.ijbiomac.2020.10.182. View

Wei Q, Duan J, Ma G, Zhang W, Wang Q, Hu Z . Enzymatic crosslinking to fabricate antioxidant peptide-based supramolecular hydrogel for improving cutaneous wound healing. J Mater Chem B. 2020; 7(13):2220-2225. DOI: 10.1039/c8tb03147a. View

Tavianatou A, Caon I, Franchi M, Piperigkou Z, Galesso D, Karamanos N . Hyaluronan: molecular size-dependent signaling and biological functions in inflammation and cancer. FEBS J. 2019; 286(15):2883-2908. DOI: 10.1111/febs.14777. View

Wang C, Chen Y, Wang Y, Liu X, Liu Y, Li Y . Inhibition of COX-2, mPGES-1 and CYP4A by isoliquiritigenin blocks the angiogenic Akt signaling in glioma through ceRNA effect of miR-194-5p and lncRNA NEAT1. J Exp Clin Cancer Res. 2019; 38(1):371. PMC: 6704644. DOI: 10.1186/s13046-019-1361-2. View

Papini R . Management of burn injuries of various depths. BMJ. 2004; 329(7458):158-60. PMC: 478230. DOI: 10.1136/bmj.329.7458.158. View

Fan C, Fu J, Zhu W, Wang D . A mussel-inspired double-crosslinked tissue adhesive intended for internal medical use. Acta Biomater. 2016; 33:51-63. DOI: 10.1016/j.actbio.2016.02.003. View

Wang C, Wang M, Xu T, Zhang X, Lin C, Gao W . Engineering Bioactive Self-Healing Antibacterial Exosomes Hydrogel for Promoting Chronic Diabetic Wound Healing and Complete Skin Regeneration. Theranostics. 2019; 9(1):65-76. PMC: 6332800. DOI: 10.7150/thno.29766. View

10.

Kamoun E, Kenawy E, Chen X . A review on polymeric hydrogel membranes for wound dressing applications: PVA-based hydrogel dressings. J Adv Res. 2017; 8(3):217-233. PMC: 5315442. DOI: 10.1016/j.jare.2017.01.005. View

11.

Yu Q, Zhang C, Jiang Z, Qin S, Zhang A . Mussel-Inspired Adhesive Polydopamine-Functionalized Hyaluronic Acid Hydrogel with Potential Bacterial Inhibition. Glob Chall. 2020; 4(2):1900068. PMC: 7001117. DOI: 10.1002/gch2.201900068. View

12.

Vig K, Chaudhari A, Tripathi S, Dixit S, Sahu R, Pillai S . Advances in Skin Regeneration Using Tissue Engineering. Int J Mol Sci. 2017; 18(4). PMC: 5412373. DOI: 10.3390/ijms18040789. View

13.

Ahmadian Z, Correia A, Hasany M, Figueiredo P, Dobakhti F, Eskandari M . A Hydrogen-Bonded Extracellular Matrix-Mimicking Bactericidal Hydrogel with Radical Scavenging and Hemostatic Function for pH-Responsive Wound Healing Acceleration. Adv Healthc Mater. 2020; 10(3):e2001122. DOI: 10.1002/adhm.202001122. View

14.

Wu Y, Wang J, Li L, Fei X, Xu L, Wang Y . A novel hydrogel with self-healing property and bactericidal activity. J Colloid Interface Sci. 2020; 584:484-494. DOI: 10.1016/j.jcis.2020.09.105. View

15.

Ahn B . Perspectives on Mussel-Inspired Wet Adhesion. J Am Chem Soc. 2017; 139(30):10166-10171. DOI: 10.1021/jacs.6b13149. View

16.

Wang X, Liu P, Wu Q, Zheng Z, Xie M, Chen G . Sustainable Antibacterial and Anti-Inflammatory Silk Suture with Surface Modification of Combined-Therapy Drugs for Surgical Site Infection. ACS Appl Mater Interfaces. 2022; 14(9):11177-11191. DOI: 10.1021/acsami.2c00106. View

17.

Ying H, Zhou J, Wang M, Su D, Ma Q, Lv G . In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing. Mater Sci Eng C Mater Biol Appl. 2019; 101:487-498. DOI: 10.1016/j.msec.2019.03.093. View

18.

Koivusalo L, Kauppila M, Samanta S, Parihar V, Ilmarinen T, Miettinen S . Tissue adhesive hyaluronic acid hydrogels for sutureless stem cell delivery and regeneration of corneal epithelium and stroma. Biomaterials. 2019; 225:119516. DOI: 10.1016/j.biomaterials.2019.119516. View

19.

Campomanes P, Rothlisberger U, Alfonso-Prieto M, Rovira C . The Molecular Mechanism of the Catalase-like Activity in Horseradish Peroxidase. J Am Chem Soc. 2015; 137(34):11170-8. DOI: 10.1021/jacs.5b06796. View

20.

Abbasi A, Sohail M, Usman Minhas M, Khaliq T, Kousar M, Khan S . Bioinspired sodium alginate based thermosensitive hydrogel membranes for accelerated wound healing. Int J Biol Macromol. 2020; 155:751-765. DOI: 10.1016/j.ijbiomac.2020.03.248. View