In Vitro Anti-Inflammatory Activity of Peptides Obtained by Tryptic Shaving of Surface Proteins of LMD-9

Overview

Journal Foods

Specialty Biotechnology

Date 2022 Apr 23

PMID 35454744

Authors

Rania Allouche

Zeeshan Hafeez

Florent Papier

Annie Dary-Mourot

Magali Genay

Laurent Miclo

Affiliations

Soon will be listed here.

Abstract

, a lactic acid bacterium widely used in the dairy industry, is consumed regularly by a significant proportion of the population. Some strains show in vitro anti-inflammatory activity which is not fully understood. We hypothesized that peptides released from the surface proteins of this bacterium during digestion could be implied in this activity. Consequently, we prepared a peptide hydrolysate by shaving and hydrolysis of surface proteins using trypsin, and the origin of peptides was checked by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Most of the identified peptides originated from bacterial cell surface proteins. The anti-inflammatory activity of peptide hydrolysate was investigated under inflammatory conditions in two cell models. Peptide hydrolysate significantly decreased secretion of pro-inflammatory cytokine IL-8 in lipopolysaccharide (LPS)-stimulated human colon epithelial HT-29 cells. It also reduced the production of pro-inflammatory cytokines IL-8, IL-1β and the protein expression levels of Pro-IL-1β and COX-2 in LPS-stimulated THP-1 macrophages. The results showed that peptides released from bacterial surface proteins by a pancreatic protease could therefore participate in an anti-inflammatory activity of LMD-9 and could prevent low-grade inflammation.

Citing Articles

: A Source of Postbiotics Displaying Anti-Inflammatory Effects in THP 1 Macrophages.

Allouche R, Hafeez Z, Dary-Mourot A, Genay M, Miclo L Molecules. 2024; 29(7).

PMID: 38611831 PMC: 11013757. DOI: 10.3390/molecules29071552.

Cell Proteins Obtained by Peptic Shaving of Two Phenotypically Different Strains of as a Source of Anti-Inflammatory Peptides.

Allouche R, Genay M, Dary-Mourot A, Hafeez Z, Miclo L Nutrients. 2022; 14(22).

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