Proteomic Response of Spores Under High Pressure Combined with Moderate Temperature and Random Peptide Mixture LK Treatment

Overview

Journal Foods

Specialty Biotechnology

Date 2022 Apr 23

PMID 35454710

Authors

Yaru Pang

Ruobin Wu

Tianlin Cui

Zequn Zhang

Li Dong

Fang Chen

Xiaosong Hu

Affiliations

Soon will be listed here.

Abstract

In this study, a method of spore inactivation under high pressure (P, 200 MPa) combined with moderate temperature (T, 80 °C) and the addition of antimicrobial peptide LK (10 μg/mL) was investigated. Spores presented cortex hydrolysis and inner membrane (IM) damage with an 8.16 log reduction in response to treatment with PT-LK, as observed by phase-contrast and inverted fluorescence microscopy and flow cytometry (FCM) analysis. Furthermore, a tandem mass tag (TMT) quantitative proteomics approach was utilized because Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis data were used. After treatment with PT-LK, 17,017 polypeptides and 3166 proteins were detected from spores. Among them, 78 proteins showed significant differences in abundance between the PT-LK-treated and control groups, with 49 proteins being upregulated and 29 proteins being downregulated in the PT-LK-treated group. Genetic information processing, metabolism, cellular process, and environmental information processing were the main mechanisms of PT-LK-mediated spore inactivation.

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