Utilizing Dual Carriers Assisted by Enzyme Digestion Chemiluminescence Signal Enhancement Strategy Simultaneously Detect Tumor Markers CEA and AFP

Overview

Journal Anal Sci

Publisher Springer

Specialty Chemistry

Date 2022 Apr 11

PMID 35403957

Authors

Xiluan Yan

Kun Zhao

Yunting Yang

Aojun Qiu

Xinlei Zhang

Jie Liu

Chengyi Zha

Xi Mai

Fanrong Ai

Xiangjuan Zheng

Affiliations

Soon will be listed here.

Abstract

To measure two tumor biomarkers, alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA), a dual-carrier CL sensor with restriction enzyme digestion (Exo I) and aptamer technology utilizing gold nanoparticles (hydroxylamine amplification) and horseradish peroxidase (HRP) as the CL signal enhancement in the sensing strategy was formed. These nanoparticles and nano-enzyme were precisely detected and tagged to the appropriate position attributable to the particular recognition of biotin and streptavidin. In this sensing strategy, target markers were further enriched and recognized sensitively by CL following enrichment, and matching strong chemical signals were collected under luminol catalysis, allowing for marker identification. For CEA (0.1-80 ng/mL) and AFP (2-500 ng/mL), the proposed method has a large linear range, with detection limits of 36.6 pg/mL and 0.94 ng/mL, respectively.

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References

Li H, Cao Z, Zhang Y, Lau C, Lu J . Simultaneous detection of two lung cancer biomarkers using dual-color fluorescence quantum dots. Analyst. 2011; 136(7):1399-405. DOI: 10.1039/c0an00704h. View

Chikkaveeraiah B, Bhirde A, Morgan N, Eden H, Chen X . Electrochemical immunosensors for detection of cancer protein biomarkers. ACS Nano. 2012; 6(8):6546-61. PMC: 3429657. DOI: 10.1021/nn3023969. View

He X, Xie X, Shao X, Song Z . Sensitive determination of gentiopicroside in medicine and bio-fluids using luminol-myoglobin chemiluminescence combined with flow injection technique. Luminescence. 2009; 25(5):384-8. DOI: 10.1002/bio.1164. View

Miao W, Bard A . Electrogenerated chemiluminescence. 77. DNA hybridization detection at high amplification with [Ru(bpy)3]2+-containing microspheres. Anal Chem. 2004; 76(18):5379-86. DOI: 10.1021/ac0495236. View

Kuhara M, Takeyama H, Tanaka T, Matsunaga T . Magnetic cell separation using antibody binding with protein a expressed on bacterial magnetic particles. Anal Chem. 2004; 76(21):6207-13. DOI: 10.1021/ac0493727. View

Bi S, Yan Y, Yang X, Zhang S . Gold nanolabels for new enhanced chemiluminescence immunoassay of alpha-fetoprotein based on magnetic beads. Chemistry. 2009; 15(18):4704-9. DOI: 10.1002/chem.200801722. View

Hai Z, Li J, Wu J, Xu J, Liang G . Alkaline Phosphatase-Triggered Simultaneous Hydrogelation and Chemiluminescence. J Am Chem Soc. 2017; 139(3):1041-1044. DOI: 10.1021/jacs.6b11041. View

Niazov A, Freeman R, Girsh J, Willner I . Following glucose oxidase activity by chemiluminescence and chemiluminescence resonance energy transfer (CRET) processes involving enzyme-DNAzyme conjugates. Sensors (Basel). 2012; 11(11):10388-97. PMC: 3274290. DOI: 10.3390/s111110388. View

Kim S, Lee Y, Shin H, Lee J . Highly sensitive chemiluminescence enzyme immunoassay for the quantification of carcinoembryonic antigen in the presence of an enhancer and a stabilizer. J Immunol Methods. 2019; 471:18-26. DOI: 10.1016/j.jim.2019.05.003. View

10.

Sun L, Zhao Q . Competitive horseradish peroxidase-linked aptamer assay for sensitive detection of Aflatoxin B1. Talanta. 2018; 179:344-349. DOI: 10.1016/j.talanta.2017.11.048. View

11.

Chen D, Feng H, Li J . Graphene oxide: preparation, functionalization, and electrochemical applications. Chem Rev. 2012; 112(11):6027-53. DOI: 10.1021/cr300115g. View

12.

Zong C, Wang R, Jiang F, Zhang D, Yang H, Wang J . Metal enhanced chemiluminescence nanosensor for ultrasensitive bioassay based on silver nanoparticles modified functional DNA dendrimer. Anal Chim Acta. 2021; 1165:338541. DOI: 10.1016/j.aca.2021.338541. View

13.

Liu C, Li B . Silver nanoparticle-initiated chemiluminescence reaction of luminol-AgNO3 and its analytical application. Anal Bioanal Chem. 2011; 401(1):229-35. DOI: 10.1007/s00216-011-5071-7. View

14.

Wang Z, Dong B, Cui X, Fan Q, Huan Y, Shan H . Core-shell Au@Pt Nanoparticles Catalyzed Luminol Chemiluminescence for Sensitive Detection of Thiocyanate. Anal Sci. 2020; 36(9):1045-1051. DOI: 10.2116/analsci.19P475. View

15.

Sun Y, Zang L, Lau C, Zhang X, Lu J . Sensitive detection of transcription factor by coupled fluorescence-encoded microsphere with exonuclease protection. Talanta. 2021; 229:122272. DOI: 10.1016/j.talanta.2021.122272. View