Dental Tissue Remineralization by Bioactive Calcium Phosphate Nanoparticles Formulations

Overview

Journal Sci Rep

Specialty Science

Date 2022 Apr 10

PMID 35397624

Authors

Andrei Cristian Ionescu

Lorenzo Degli Esposti

Michele Iafisco

Eugenio Brambilla

Affiliations

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Abstract

Recent health care products are based on formulations claimed to provide enamel remineralization and dentinal tubules occlusion through calcium-phosphate bioactive nanocompounds (ion-doped hydroxyapatite and precursor, amorphous calcium phosphate nanoparticles). This study aimed to characterize, test, and compare for the first time the structure and performance of a representative, market-available sample of remineralizing toothpastes and topical mousses. Formulations were characterized to determine their composition and investigate the presence of bioactive compounds and doping elements. A conventional fluoride-containing toothpaste was used as reference. The enamel remineralization and efficacy of dentinal tubules occlusion by tested formulations were investigated ex vivo on human hard tissues. All formulations containing Ca-P bioactive nanocompounds showed remineralizing ability by epitaxial growth of a layer showing the morphology and composition of human hydroxyapatite. Such layers also embedded nanosilica clusters. The presence of doping elements or casein phosphopeptide seemed essential to allow such performances, especially when hydroxyapatite and amorphous calcium phosphate compounds were doped with small amounts of CO, F, Mg, and Sr. Topical mousse formulations showed a higher tubules occlusion capability than toothpastes, independently from their composition. Therefore, all tested formulations could be useful in restoring tooth structures in a biomimetic way, contrasting dental demineralization processes leading to caries.

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References

Saito T, Toyooka H, Ito S, Crenshaw M . In vitro study of remineralization of dentin: effects of ions on mineral induction by decalcified dentin matrix. Caries Res. 2003; 37(6):445-9. DOI: 10.1159/000073398. View

Tschoppe P, Zandim D, Martus P, Kielbassa A . Enamel and dentine remineralization by nano-hydroxyapatite toothpastes. J Dent. 2011; 39(6):430-7. DOI: 10.1016/j.jdent.2011.03.008. View

Robinson C, Shore R, Wood S, Brookes S, Smith D, Wright J . Subunit structures in hydroxyapatite crystal development in enamel: implications for amelogenesis imperfecta. Connect Tissue Res. 2003; 44 Suppl 1:65-71. View

Lacruz R, Habelitz S, Wright J, Paine M . DENTAL ENAMEL FORMATION AND IMPLICATIONS FOR ORAL HEALTH AND DISEASE. Physiol Rev. 2017; 97(3):939-993. PMC: 6151498. DOI: 10.1152/physrev.00030.2016. View

Garcia-Godoy F, Hicks M . Maintaining the integrity of the enamel surface: the role of dental biofilm, saliva and preventive agents in enamel demineralization and remineralization. J Am Dent Assoc. 2008; 139 Suppl:25S-34S. DOI: 10.14219/jada.archive.2008.0352. View

Li J, Xie X, Wang Y, Yin W, Antoun J, Farella M . Long-term remineralizing effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) on early caries lesions in vivo: a systematic review. J Dent. 2014; 42(7):769-77. DOI: 10.1016/j.jdent.2014.03.015. View

Farooq I, Bugshan A . The role of salivary contents and modern technologies in the remineralization of dental enamel: a narrative review. F1000Res. 2021; 9:171. PMC: 7076334. DOI: 10.12688/f1000research.22499.3. View

Yoshiyama M, Masada J, Uchida A, Ishida H . Scanning electron microscopic characterization of sensitive vs. insensitive human radicular dentin. J Dent Res. 1989; 68(11):1498-502. DOI: 10.1177/00220345890680110601. View

Maguire A . ADA clinical recommendations on topical fluoride for caries prevention. Evid Based Dent. 2014; 15(2):38-9. DOI: 10.1038/sj.ebd.6401019. View

10.

Hellwig E, Polydorou O, Lussi A, Kielbassa A, Altenburger M . The influence of saliva on the dissolution of calcium fluoride after application of different fluoride gels in vitro. Quintessence Int. 2010; 41(9):773-7. View

11.

Ionescu A, Cazzaniga G, Ottobelli M, Garcia-Godoy F, Brambilla E . Substituted Nano-Hydroxyapatite Toothpastes Reduce Biofilm Formation on Enamel and Resin-Based Composite Surfaces. J Funct Biomater. 2020; 11(2). PMC: 7353493. DOI: 10.3390/jfb11020036. View

12.

Kielbassa A, Wrbas K, Schulte-Monting J, Hellwig E . Correlation of transversal microradiography and microhardness on in situ-induced demineralization in irradiated and nonirradiated human dental enamel. Arch Oral Biol. 1999; 44(3):243-51. DOI: 10.1016/s0003-9969(98)00123-x. View

13.

Geng Z, Cui Z, Li Z, Zhu S, Liang Y, Lu W . Synthesis, characterization and the formation mechanism of magnesium- and strontium-substituted hydroxyapatite. J Mater Chem B. 2020; 3(18):3738-3746. DOI: 10.1039/c4tb02148g. View

14.

Liu Y, Lee Y, Huang T, Lin G, Chen Y, Lee B . In Vitro Bioactivity and Antibacterial Activity of Strontium-, Magnesium-, and Zinc-Multidoped Hydroxyapatite Porous Coatings Applied via Atmospheric Plasma Spraying. ACS Appl Bio Mater. 2022; 4(3):2523-2533. DOI: 10.1021/acsabm.0c01535. View

15.

Degli Esposti L, Ionescu A, Brambilla E, Tampieri A, Iafisco M . Characterization of a Toothpaste Containing Bioactive Hydroxyapatites and In Vitro Evaluation of Its Efficacy to Remineralize Enamel and to Occlude Dentinal Tubules. Materials (Basel). 2020; 13(13). PMC: 7372420. DOI: 10.3390/ma13132928. View

16.

Guimaraes I, Rezende C, da Silva J, Jesus D . Simultaneous determination of free fluoride and monofluorophosphate in toothpaste by capillary electrophoresis with capacitively coupled contactless conductivity detection. Talanta. 2009; 78(4-5):1436-9. DOI: 10.1016/j.talanta.2009.02.041. View

17.

Fernandez-Ferrer L, Vicente-Ruiz M, Garcia-Sanz V, Montiel-Company J, Paredes-Gallardo V, Almerich-Silla J . Enamel remineralization therapies for treating postorthodontic white-spot lesions: A systematic review. J Am Dent Assoc. 2018; 149(9):778-786.e2. DOI: 10.1016/j.adaj.2018.05.010. View

18.

Iafisco M, Degli Esposti L, Ramirez-Rodriguez G, Carella F, Gomez-Morales J, Ionescu A . Fluoride-doped amorphous calcium phosphate nanoparticles as a promising biomimetic material for dental remineralization. Sci Rep. 2018; 8(1):17016. PMC: 6242929. DOI: 10.1038/s41598-018-35258-x. View

19.

Ionescu A, Comba A, Brambilla E, Ilie N, Breschi L, Cadenaro M . Influence of Curing Time on the Microbiological Behavior of Bulk-Fill Nanohybrid Resin Composites. Polymers (Basel). 2021; 13(17). PMC: 8434565. DOI: 10.3390/polym13172948. View

20.

Lechien J, Calvo-Henriquez C, Chiesa-Estomba C, Barillari M, Trozzi M, Meucci D . Reflux and dental disorders in the pediatric population: A systematic review. Int J Pediatr Otorhinolaryngol. 2020; 136:110166. DOI: 10.1016/j.ijporl.2020.110166. View