Studying Porifera WBR Using the Calcerous Sponges Leucosolenia

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2022 Apr 1

PMID 35359303

Authors

Andrey I Lavrov

Alexander V Ereskovsky

Affiliations

Soon will be listed here.

Abstract

Sponges (Porifera), basal nonbilaterian metazoans, are well known for their high regenerative capacities ranging from reparation of a lost body wall to whole-body regeneration from a small piece of tissues or even from dissociated cells. Sponges from different clades utilize different cell sources and various morphological processes to complete the regeneration. This variety makes these animals promising models for studying the evolution of regeneration in Metazoa. However, there are few publications concerning the regenerative mechanisms in sponges. This could be partially explained by the delicacy of sponge tissues, which requires modifying and fine adjusting of common research protocols. The current chapter describes various methods for studying regeneration processes in the marine calcareous sponge, Leucosolenia. Provided protocols span all significant research steps: from sponge collection and surgical operations to various types of microscopy and immunohistochemical studies.

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References

Simion P, Philippe H, Baurain D, Jager M, Richter D, Di Franco A . A Large and Consistent Phylogenomic Dataset Supports Sponges as the Sister Group to All Other Animals. Curr Biol. 2017; 27(7):958-967. DOI: 10.1016/j.cub.2017.02.031. View

Ereskovsky A, Tokina D, Saidov D, Baghdiguian S, Le Goff E, Lavrov A . Transdifferentiation and mesenchymal-to-epithelial transition during regeneration in Demospongiae (Porifera). J Exp Zool B Mol Dev Evol. 2019; 334(1):37-58. DOI: 10.1002/jez.b.22919. View

Bond C . Continuous cell movements rearrange anatomical structures in intact sponges. J Exp Zool. 1992; 263(3):284-302. DOI: 10.1002/jez.1402630308. View

Ereskovsky A, Borisenko I, Bolshakov F, Lavrov A . Whole-Body Regeneration in Sponges: Diversity, Fine Mechanisms, and Future Prospects. Genes (Basel). 2021; 12(4). PMC: 8066720. DOI: 10.3390/genes12040506. View

Lavrov A, Kosevich I . Sponge cell reaggregation: Cellular structure and morphogenetic potencies of multicellular aggregates. J Exp Zool A Ecol Genet Physiol. 2016; 325(2):158-77. DOI: 10.1002/jez.2006. View

Lavrov A, Bolshakov F, Tokina D, Ereskovsky A . Sewing up the wounds : The epithelial morphogenesis as a central mechanism of calcaronean sponge regeneration. J Exp Zool B Mol Dev Evol. 2018; 330(6-7):351-371. DOI: 10.1002/jez.b.22830. View

Kenny N, de Goeij J, de Bakker D, Whalen C, Berezikov E, Riesgo A . Towards the identification of ancestrally shared regenerative mechanisms across the Metazoa: A Transcriptomic case study in the Demosponge Halisarca caerulea. Mar Genomics. 2017; 37:135-147. DOI: 10.1016/j.margen.2017.11.001. View

Borisenko I, Adamska M, Tokina D, Ereskovsky A . Transdifferentiation is a driving force of regeneration in Halisarca dujardini (Demospongiae, Porifera). PeerJ. 2015; 3:e1211. PMC: 4556153. DOI: 10.7717/peerj.1211. View

Borisenko I, Adamski M, Ereskovsky A, Adamska M . Surprisingly rich repertoire of Wnt genes in the demosponge Halisarca dujardini. BMC Evol Biol. 2016; 16(1):123. PMC: 4902976. DOI: 10.1186/s12862-016-0700-6. View

10.

Ereskovsky A, Borisenko I, Lapebie P, Gazave E, Tokina D, Borchiellini C . Oscarella lobularis (Homoscleromorpha, Porifera) Regeneration: Epithelial Morphogenesis and Metaplasia. PLoS One. 2015; 10(8):e0134566. PMC: 4536211. DOI: 10.1371/journal.pone.0134566. View

11.

KOROTKOVA G . Behaviour of the cellular elements in the calcareous sponge Leucosolenia complicata mont, during regeneration. Acta Biol Acad Sci Hung. 1962; 13:1-30. View

12.

KOROTKOVA G . [Peculiarities of morphogenesis in the development of the calcareous sponge Leucosolenia complicata Mont. from small parts of its body wall]. Vestn Leningr Univ Biol. 1969; 3:15-22. View

13.

Alexander B, Achlatis M, Osinga R, van der Geest H, Cleutjens J, Schutte B . Cell kinetics during regeneration in the sponge Halisarca caerulea: how local is the response to tissue damage?. PeerJ. 2015; 3:e820. PMC: 4358696. DOI: 10.7717/peerj.820. View

14.

Morganti T, Ribes M, Yahel G, Coma R . Size Is the Major Determinant of Pumping Rates in Marine Sponges. Front Physiol. 2020; 10:1474. PMC: 6917621. DOI: 10.3389/fphys.2019.01474. View