Digital Plasmonic Nanobubble Detection for Rapid and Ultrasensitive Virus Diagnostics

Overview

Journal Nat Commun

Specialty Biology

Date 2022 Mar 31

PMID 35354801

Authors

Yaning Liu

Haihang Ye

HoangDinh Huynh

Chen Xie

Peiyuan Kang

Jeffrey S Kahn

Zhenpeng Qin

Affiliations

Soon will be listed here.

Abstract

Rapid and sensitive diagnostics of infectious diseases is an urgent and unmet need as evidenced by the COVID-19 pandemic. Here, we report a strategy, based on DIgitAl plasMONic nanobubble Detection (DIAMOND), to address this need. Plasmonic nanobubbles are transient vapor bubbles generated by laser heating of plasmonic nanoparticles (NPs) and allow single-NP detection. Using gold NPs as labels and an optofluidic setup, we demonstrate that DIAMOND achieves compartment-free digital counting and works on homogeneous immunoassays without separation and amplification steps. DIAMOND allows specific detection of respiratory syncytial virus spiked in nasal swab samples and achieves a detection limit of ~100 PFU/mL (equivalent to 1 RNA copy/µL), which is competitive with digital isothermal amplification for virus detection. Therefore, DIAMOND has the advantages including one-step and single-NP detection, direct sensing of intact viruses at room temperature, and no complex liquid handling, and is a platform technology for rapid and ultrasensitive diagnostics.

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