Multiple Independent Control Mechanisms Affecting the Acetamidase of Aspergillus Nidulans

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1978 Apr 25

PMID 353500

Citations 26

Authors

M J Hynes

Affiliations

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Abstract

The acetamidase of Aspergillus nidulans is induced by sources of acetyl CoA, benzoate and benzamide and by beta-alanine and other omega-amino acids. The effects of these groups of inducers are appromimately additive. The cis-acting control site mutant, amdI9, affects induction by sources of acetyl-CoA specifically. Lesions in the amdR and gatA genes affect induction by omega-amino acids specifically. Mutations in the amdA gene can lead to elevated acetamidase levels which still respond to the various inducers. The induction controls act independently of repression control by nitrogen metabolites and are not altered by the areA102 mutation. The properties of double mutants with lesions affecting the different control mechanisms also indicate their independence of each other. It is suggested that the acetamidase is subject to complex control by multiple regulatory circuits and that functionally independent control sites adjacent to the structural gene occur.

Citing Articles

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A paradoxical mutant GATA factor.

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Subtle hydrophobic interactions between the seventh residue of the zinc finger loop and the first base of an HGATAR sequence determine promoter-specific recognition by the Aspergillus nidulans GATA factor AreA.

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The cloning and sequencing of the alcB gene, coding for alcohol dehydrogenase II, in Aspergillus nidulans.

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