ALDH2 Expression, Alcohol Intake and Semen Parameters Among East Asian Men

Overview

Journal J Urol

Publisher Wolters Kluwer

Specialty Urology

Date 2022 Mar 28

PMID 35344413

Authors

Daniel R Greenberg

Hriday P Bhambhvani

Satvir S Basran

Brett P Salazar

Luis Carl Rios

Sin-Jin Li

Che-Hong Chen

Daria Mochly-Rosen

Michael L Eisenberg

Affiliations

Soon will be listed here.

Abstract

Purpose: Inactivating mutations in mitochondrial aldehyde dehydrogenase 2 (ALDH2) are highly prevalent. The most common variant allele, , is present in 40%-50% of East Asians, and causes acetaldehyde accumulation, flushing and tachycardia after alcohol intake. The relationship between alcohol intake and ALDH2 genotype on semen parameters remains unknown.

Materials And Methods: We conducted a cross-sectional study to determine the association between ALDH2 genotype, alcohol consumption and semen parameters among East Asian men. Volunteers completed a survey and submitted a semen sample for analysis. Participants were genotyped to determine ALDH2 status (, , ), and immunohistochemical staining was used to determine protein expression of ALDH2 in spermatozoa.

Results: Of 112 men 45 (40.2%) were carriers. Among carriers, alcohol consumption was associated with significantly lower total sperm motility (median 20% [interquartile range 11%-42%] vs 43% [IQR 31%-57%], p=0.005) and progressive sperm motility (19% [IQR 11%-37%] vs 36% [IQR 25%-53%], p=0.008). Among alcohol consumers, carriers had significantly lower total sperm motility (20% [IQR 11%--42%] vs 41% [IQR 19%-57%], p=0.02), progressive sperm motility (19% [IQR 11%-37%] vs 37% [IQR 17%-50%], p=0.02) and total motile sperm count (28 million [M; IQR 9-79M] vs 71M [IQR 23-150M], p=0.05) compared to individuals. Secondly, ALDH2 expression in human spermatozoa was significantly lower in carriers ( vs , p=0.01; vs , p <0.001).

Conclusions: Our findings suggest genotyping ALDH2, coupled with alcohol cessation counseling, may improve semen parameters among men.

Citing Articles

Advances in Factors Affecting ALDH2 Activity and its Mechanisms.

Liu Y, Liu X, Pan C Cardiovasc Toxicol. 2024; 24(12):1428-1438.

PMID: 39365551 DOI: 10.1007/s12012-024-09923-9.

[Association analysis between mixed exposure to phenols and semen quality].

Shen X, Tang W, Liu J, Ao J, Liu X, Huang X Se Pu. 2024; 42(2):203-210.

PMID: 38374601 PMC: 10877479. DOI: 10.3724/SP.J.1123.2023.09009.

Association of diet and lifestyle factors with semen quality in male partners of Chinese couples preparing for pregnancy.

Mai H, Ke J, Zheng Z, Luo J, Li M, Qu Y Reprod Health. 2023; 20(1):173.

PMID: 37996913 PMC: 10666430. DOI: 10.1186/s12978-023-01718-5.

Male infertility.

Eisenberg M, Esteves S, Lamb D, Hotaling J, Giwercman A, Hwang K Nat Rev Dis Primers. 2023; 9(1):49.

PMID: 37709866 DOI: 10.1038/s41572-023-00459-w.

[Aldehyde dehydrogenase 2 rs671 genetic polymorphisms are associated with chemotherapy-induced nausea and vomiting].

Yang Q, Liu X, Jiang Y, Ma J Nan Fang Yi Ke Da Xue Xue Bao. 2023; 43(6):1017-1022.

PMID: 37439175 PMC: 10339303. DOI: 10.12122/j.issn.1673-4254.2023.06.18.

References

Pajarinen J, Savolainen V, Perola M, Penttila A, Karhunen P . Polymorphism in the cytochrome P450 2E1 gene and alcohol-induced disorders of human spermatogenesis. Int J Androl. 1996; 19(5):314-22. DOI: 10.1111/j.1365-2605.1996.tb00482.x. View

Xu H, Zhang Y, Ren J . ALDH2 and Stroke: A Systematic Review of the Evidence. Adv Exp Med Biol. 2019; 1193:195-210. DOI: 10.1007/978-981-13-6260-6_11. View

Li H, Borinskaya S, Yoshimura K, Kalina N, Marusin A, Stepanov V . Refined geographic distribution of the oriental ALDH2*504Lys (nee 487Lys) variant. Ann Hum Genet. 2009; 73(Pt 3):335-45. PMC: 2846302. DOI: 10.1111/j.1469-1809.2009.00517.x. View

Oyama T, Isse T, Kagawa N, Kinaga T, Kim Y, Morita M . Tissue-distribution of aldehyde dehydrogenase 2 and effects of the ALDH2 gene-disruption on the expression of enzymes involved in alcohol metabolism. Front Biosci. 2004; 10:951-60. DOI: 10.2741/1589. View

Khandwala Y, Zhang C, Li S, Behr B, Guo D, Eisenberg M . Racial Variation in Semen Quality at Fertility Evaluation. Urology. 2017; 106:96-102. DOI: 10.1016/j.urology.2017.03.064. View

Chen C, Lu R, Chen Y, Wang M, Chang Y, Li T . Interaction between the functional polymorphisms of the alcohol-metabolism genes in protection against alcoholism. Am J Hum Genet. 1999; 65(3):795-807. PMC: 1377988. DOI: 10.1086/302540. View

Anderson J, Farr S, Jamieson D, Warner L, Macaluso M . Infertility services reported by men in the United States: national survey data. Fertil Steril. 2008; 91(6):2466-70. DOI: 10.1016/j.fertnstert.2008.03.022. View

Yang S, Lee J, Choi I, Kim Y, Ryu K, Sung J . Effects of alcohol consumption, ALDH2 rs671 polymorphism, and Helicobacter pylori infection on the gastric cancer risk in a Korean population. Oncotarget. 2016; 8(4):6630-6641. PMC: 5351658. DOI: 10.18632/oncotarget.14250. View

Sharma R, Harlev A, Agarwal A, Esteves S . Cigarette Smoking and Semen Quality: A New Meta-analysis Examining the Effect of the 2010 World Health Organization Laboratory Methods for the Examination of Human Semen. Eur Urol. 2016; 70(4):635-645. DOI: 10.1016/j.eururo.2016.04.010. View

10.

Eriksson C . The role of acetaldehyde in the actions of alcohol (update 2000). Alcohol Clin Exp Res. 2001; 25(5 Suppl ISBRA):15S-32S. DOI: 10.1097/00000374-200105051-00005. View

11.

Chang J, Hsiao J, Chen C . ALDH2 polymorphism and alcohol-related cancers in Asians: a public health perspective. J Biomed Sci. 2017; 24(1):19. PMC: 5335829. DOI: 10.1186/s12929-017-0327-y. View

12.

Hoyer S, Riis A, Toft G, Wise L, Hatch E, Wesselink A . Male alcohol consumption and fecundability. Hum Reprod. 2020; 35(4):816-825. PMC: 7192537. DOI: 10.1093/humrep/dez294. View

13.

La Vignera S, Condorelli R, Balercia G, Vicari E, Calogero A . Does alcohol have any effect on male reproductive function? A review of literature. Asian J Androl. 2013; 15(2):221-5. PMC: 3739141. DOI: 10.1038/aja.2012.118. View

14.

Lai C, Yao C, Chau G, Yang L, Kuo T, Chiang C . Dominance of the inactive Asian variant over activity and protein contents of mitochondrial aldehyde dehydrogenase 2 in human liver. Alcohol Clin Exp Res. 2013; 38(1):44-50. DOI: 10.1111/acer.12215. View

15.

Chen Y, Peng G, Tsao T, Wang M, Lu R, Yin S . Pharmacokinetic and pharmacodynamic basis for overcoming acetaldehyde-induced adverse reaction in Asian alcoholics, heterozygous for the variant ALDH2*2 gene allele. Pharmacogenet Genomics. 2009; 19(8):588-99. DOI: 10.1097/FPC.0b013e32832ecf2e. View

16.

Evers J . Female subfertility. Lancet. 2002; 360(9327):151-9. DOI: 10.1016/S0140-6736(02)09417-5. View

17.

Glazer C, Li S, Zhang C, Giwercman A, Bonde J, Eisenberg M . Racial and Sociodemographic Differences of Semen Parameters Among US Men Undergoing a Semen Analysis. Urology. 2018; 123:126-132. DOI: 10.1016/j.urology.2018.09.029. View

18.

Malkovskiy A, Van Wassenhove L, Goltsev Y, Osei-Sarfo K, Chen C, Efron B . The Effect of Ethanol Consumption on Composition and Morphology of Femur Cortical Bone in Wild-Type and ALDH2*2-Homozygous Mice. Calcif Tissue Int. 2020; 108(2):265-276. PMC: 8092984. DOI: 10.1007/s00223-020-00769-1. View

19.

Chen J, Huang W, Cheng C, Zhou L, Jiang G, Hu Y . Association Between Polymorphisms and Risk of Alzheimer's Disease and Parkinson's Disease: A Meta-Analysis Based on 5,315 Individuals. Front Neurol. 2019; 10:290. PMC: 6448532. DOI: 10.3389/fneur.2019.00290. View

20.

Nassan F, Chavarro J, Tanrikut C . Diet and men's fertility: does diet affect sperm quality?. Fertil Steril. 2018; 110(4):570-577. DOI: 10.1016/j.fertnstert.2018.05.025. View