Sanguinarine-Chelerythrine Fraction of Exerts Anti-inflammatory Activity in Carrageenan Paw Oedema Test in Rats and Reveals Reduced Gastrotoxicity

Overview

Journal Oxid Med Cell Longev

Publisher Wiley

Specialties Cell Biology
Endocrinology

Date 2022 Mar 28

PMID 35340213

Authors

Maciej Danielewski

Sylwia Zielinska

Agnieszka Matuszewska

Wojciech Slupski

Maciej Wlodarczyk

Izabela Jeskowiak

Benita Wiatrak

Krzysztof Kowalski

Anna Jezierska-Domaradzka

Piotr Ziolkowski

Adam Szelag

Beata Nowak

Affiliations

Soon will be listed here.

Abstract

Inflammatory diseases are a common therapeutic problem and nonsteroidal anti-inflammatory drugs are not deprived of side effects, of which ulcerogenic activity is one of the most frequent. The aim of the study was to evaluate the anti-inflammatory activity of the sanguinarine-chelerythrine (SC) fraction of and its influence on the integrity of gastric mucosa. The study was conducted on sixty male rats randomly divided into six experimental groups: two control groups (a negative control group CON and a positive control group CAR); three groups receiving an investigational fraction of (1, 5, 10 mg/kg ) named SC, SC, and SC, respectively; and a group receiving indomethacin (IND) (10 mg/kg ) as a reference drug. In all animals, the carrageenan-induced paw oedema was measured; PGE release, TNF production, and MMP-9 concentration in inflamed tissue were determined. Additionally, the macroscopic and microscopic damage of gastric mucosa was evaluated. Administration of SC dose-dependently inhibited the second phase of carrageenan rat paw oedema and PGE release, decreased the production of TNF, and reduced the concentration of MMP-9, and the efficacy of the highest dose was comparable to the effect of IND. Contrary to IND, no gastrotoxic activity of SC was detected. The investigated sanguinarine-chelerythrine fraction of seems to be a promising candidate for further research on new anti-inflammatory and analgesic drugs characterized with a safer gastric profile compared to existing NSAIDs.

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