Antibacterial Activity and Mechanism of Sanguinarine Against in Vitro

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2020 Sep 1

PMID 32864203

Citations 19

Authors

Qian Zhang

Yansi Lyu

Jingkai Huang

Xiaodong Zhang

Na Yu

Ziping Wen

Si Chen

Affiliations

Soon will be listed here.

Abstract

Background: Sanguinarine (SAG), a benzophenanthridine alkaloid, occurs in , and families. Studies have found that SAG has antioxidant, anti-inflammatory, and antiproliferative activities in several malignancies and that it exhibits robust antibacterial activities. However, information reported on the action of SAG against is limited in the literature. Therefore, the present study aimed to evaluate the antimicrobial and antibiofilm activities of SAG against in vitro.

Methods: The agar dilution method was used to determine the minimum inhibitory concentration (MIC) of SAG against . The intracellular ATP concentration, intracellular pH (pH), and cell membrane integrity and potential were measured. Confocal laser scanning microscopy (CLSM), field emission scanning electron microscopy (FESEM), and crystal violet staining were used to measure the antibiofilm formation of SAG.

Results: The MIC of SAG against was 7.8 μg/mL. SAG inhibited the growth of and destroyed the integrity of cell membrane, as reflected mainly through the decreases in the intracellular ATP concentration, pH and cell membrane potential and significant changes in cellular morphology. The findings of CLSM, FESEM and crystal violet staining indicated that SAG exhibited strong inhibitory effects on the biofilm formation of and led to the inactivity of biofilm-related cells.

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