Kinetics of Placental Infection by Different Smooth Strains in Mice

Overview

Journal Pathogens

Date 2022 Mar 26

PMID 35335603

Authors

Irati Poveda-Urkixo

Gustavo A Ramirez

Maria-Jesus Grillo

Affiliations

Soon will be listed here.

Abstract

Abortion and reproductive failures induced by are the main symptoms of animal brucellosis. Laboratory animal models are essential tools of research to study the pathogenesis before experimentation in natural hosts. To extend the existing knowledge, we studied 16M (virulent) and Rev1 (attenuated) as well as bv2 infections in pregnant mice. Here, we report new information about kinetics of infection (in spleens, blood, placentas, vaginal shedding, and foetuses), serum cytokine profiles, and histopathological features in placentas and the litter throughout mice pregnancy. Both strains showed a marked placental tropism and reduced viability of pups (mainly in 16M infections), which was preceded by an intense Th1-immune response during placental development. In contrast, bv2 displayed lower placental tropism, mild proinflammatory immune response, and scarce bacterial transmission to the litter, thus allowing foetal viability. Overall, our studies revealed three different smooth patterns of placental and foetal pathogenesis in mice, providing a useful animal model for experimental brucellosis.

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References

Dorneles E, Teixeira-Carvalho A, Araujo M, Sriranganathan N, Lage A . Immune response triggered by Brucella abortus following infection or vaccination. Vaccine. 2015; 33(31):3659-66. DOI: 10.1016/j.vaccine.2015.05.057. View

Tobias L, Cordes D, Schurig G . Placental pathology of the pregnant mouse inoculated with Brucella abortus strain 2308. Vet Pathol. 1993; 30(2):119-29. DOI: 10.1177/030098589303000204. View

Zhan Y, Cheers C . Endogenous interleukin-12 is involved in resistance to Brucella abortus infection. Infect Immun. 1995; 63(4):1387-90. PMC: 173163. DOI: 10.1128/iai.63.4.1387-1390.1995. View

Whiteside E, Boucaut K, Teh A, Garcia-Aragon J, Harvey M, Herington A . Elevated concentration of TNF-alpha induces trophoblast differentiation in mouse blastocyst outgrowths. Cell Tissue Res. 2003; 314(2):275-80. DOI: 10.1007/s00441-003-0791-4. View

Barber E, Fazzari M, Pollard J . Th1 cytokines are essential for placental immunity to Listeria monocytogenes. Infect Immun. 2005; 73(10):6322-31. PMC: 1230899. DOI: 10.1128/IAI.73.10.6322-6331.2005. View

Detilleux P, DEYOE B, Cheville N . Penetration and intracellular growth of Brucella abortus in nonphagocytic cells in vitro. Infect Immun. 1990; 58(7):2320-8. PMC: 258815. DOI: 10.1128/iai.58.7.2320-2328.1990. View

Duran-Ferrer M, Leon L, Nielsen K, Caporale V, Mendoza J, Osuna A . Antibody response and antigen-specific gamma-interferon profiles of vaccinated and unvaccinated pregnant sheep experimentally infected with Brucella melitensis. Vet Microbiol. 2004; 100(3-4):219-31. DOI: 10.1016/j.vetmic.2004.02.008. View

Kim S, Lee D, Watanabe K, Furuoka H, Suzuki H, Watarai M . Interferon-gamma promotes abortion due to Brucella infection in pregnant mice. BMC Microbiol. 2005; 5:22. PMC: 1090583. DOI: 10.1186/1471-2180-5-22. View

Garcia-Mendez K, Hielpos S, Soler-Llorens P, Arce-Gorvel V, Hale C, Gorvel J . Infection by Brucella melitensis or Brucella papionis modifies essential physiological functions of human trophoblasts. Cell Microbiol. 2019; 21(7):e13019. DOI: 10.1111/cmi.13019. View

10.

Whatmore A . Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens. Infect Genet Evol. 2009; 9(6):1168-84. DOI: 10.1016/j.meegid.2009.07.001. View

11.

de Miguel M, Marin C, Munoz P, Dieste L, Grillo M, Blasco J . Development of a selective culture medium for primary isolation of the main Brucella species. J Clin Microbiol. 2011; 49(4):1458-63. PMC: 3122841. DOI: 10.1128/JCM.02301-10. View

12.

Guimaraes E, Martins J, Gomes M, Cerqueira D, Oliveira S . Lack of Interleukin-6 Affects IFN-γ and TNF-α Production and Early In Vivo Control of Infection. Pathogens. 2020; 9(12). PMC: 7764695. DOI: 10.3390/pathogens9121040. View

13.

Lalsiamthara J, Lee J . Development and trial of vaccines against . J Vet Sci. 2017; 18(S1):281-290. PMC: 5583415. DOI: 10.4142/jvs.2017.18.S1.281. View

14.

Roop 2nd R, Barton I, Hopersberger D, Martin D . Uncovering the Hidden Credentials of Virulence. Microbiol Mol Biol Rev. 2021; 85(1). PMC: 8549849. DOI: 10.1128/MMBR.00021-19. View

15.

Bialer M, Ferrero M, Delpino M, Ruiz-Ranwez V, Posadas D, Baldi P . Adhesive Functions or Pseudogenization of Type Va Autotransporters in Species. Front Cell Infect Microbiol. 2021; 11:607610. PMC: 8111173. DOI: 10.3389/fcimb.2021.607610. View

16.

Athanassakis I, Iconomidou B . Cytokine production in the serum and spleen of mice from day 6 to 14 of gestation: cytokines/placenta/spleen/serum. Dev Immunol. 1996; 4(4):247-55. PMC: 2275966. DOI: 10.1155/1995/42412. View

17.

Gonzalez D, Grillo M, de Miguel M, Ali T, Arce-Gorvel V, Delrue R . Brucellosis vaccines: assessment of Brucella melitensis lipopolysaccharide rough mutants defective in core and O-polysaccharide synthesis and export. PLoS One. 2008; 3(7):e2760. PMC: 2453230. DOI: 10.1371/journal.pone.0002760. View

18.

Barbier T, Machelart A, Zuniga-Ripa A, Plovier H, Hougardy C, Lobet E . Erythritol Availability in Bovine, Murine and Human Models Highlights a Potential Role for the Host Aldose Reductase during Infection. Front Microbiol. 2017; 8:1088. PMC: 5468441. DOI: 10.3389/fmicb.2017.01088. View

19.

Raghupathy R . Th1-type immunity is incompatible with successful pregnancy. Immunol Today. 1997; 18(10):478-82. DOI: 10.1016/s0167-5699(97)01127-4. View

20.

Wang W, Sung N, Gilman-Sachs A, Kwak-Kim J . T Helper (Th) Cell Profiles in Pregnancy and Recurrent Pregnancy Losses: Th1/Th2/Th9/Th17/Th22/Tfh Cells. Front Immunol. 2020; 11:2025. PMC: 7461801. DOI: 10.3389/fimmu.2020.02025. View