Cell and Tissue Tropism of Brucella Spp

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2023 May 2

PMID 37129522

Authors

Thaynara Parente de Carvalho

Laice Alves da Silva

Thais Larissa Lourenco Castanheira

Tayse Domingues de Souza

Tatiane Alves da Paixao

Leticia Lazaro-Anton

Renee M Tsolis

Renato Lima Santos

Affiliations

Soon will be listed here.

Abstract

Brucella spp. are facultatively intracellular bacteria that can infect, survive, and multiply in various host cell types and/or . The genus Brucella has markedly expanded in recent years with the identification of novel species and hosts, which has revealed additional information about the cell and tissue tropism of these pathogens. Classically, Brucella spp. are considered to have tropism for organs that contain large populations of phagocytes such as lymph nodes, spleen, and liver, as well as for organs of the genital system, including the uterus, epididymis, testis, and placenta. However, experimental infections of several different cultured cell types indicate that Brucella may actually have a broader cell tropism than previously thought. Indeed, recent studies indicate that certain Brucella species in particular hosts may display a pantropic distribution . This review discusses the available knowledge on cell and tissue tropism of Brucella spp. in natural infections of various host species, as well as in experimental animal models and cultured cells.

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References

Scholz H, Hubalek Z, Sedlacek I, Vergnaud G, Tomaso H, Al Dahouk S . Brucella microti sp. nov., isolated from the common vole Microtus arvalis. Int J Syst Evol Microbiol. 2008; 58(Pt 2):375-82. DOI: 10.1099/ijs.0.65356-0. View

Kim S, Lee D, Watanabe K, Furuoka H, Suzuki H, Watarai M . Interferon-gamma promotes abortion due to Brucella infection in pregnant mice. BMC Microbiol. 2005; 5:22. PMC: 1090583. DOI: 10.1186/1471-2180-5-22. View

Garcia-Mendez K, Hielpos S, Soler-Llorens P, Arce-Gorvel V, Hale C, Gorvel J . Infection by Brucella melitensis or Brucella papionis modifies essential physiological functions of human trophoblasts. Cell Microbiol. 2019; 21(7):e13019. DOI: 10.1111/cmi.13019. View

Ronai Z, Kreizinger Z, Dan A, Drees K, Foster J, Banyai K . First isolation and characterization of Brucella microti from wild boar. BMC Vet Res. 2015; 11:147. PMC: 4499207. DOI: 10.1186/s12917-015-0456-z. View

Zavattieri L, Ferrero M, Alonso Paiva I, Sotelo A, Canellada A, Baldi P . Proliferates in Decidualized and Non-Decidualized Human Endometrial Cells Inducing a Proinflammatory Response. Pathogens. 2020; 9(5). PMC: 7281465. DOI: 10.3390/pathogens9050369. View

Kang Y, Kirby J . A Chemical Genetics Screen Reveals Influence of p38 Mitogen-Activated Protein Kinase and Autophagy on Phagosome Development and Intracellular Replication of Brucella neotomae in Macrophages. Infect Immun. 2019; 87(8). PMC: 6652748. DOI: 10.1128/IAI.00044-19. View

Dawson C, Perrett L, Young E, Davison N, Monies R . Isolation of Brucella species from a bottlenosed dolphin (Tursiops truncatus). Vet Rec. 2006; 158(24):831-2. DOI: 10.1136/vr.158.24.831. View

Poester F, Samartino L, Santos R . Pathogenesis and pathobiology of brucellosis in livestock. Rev Sci Tech. 2013; 32(1):105-15. DOI: 10.20506/rst.32.1.2193. View

Guzman-Verri C, Chaves-Olarte E, von Eichel-Streiber C, Lopez-Goni I, Thelestam M, Arvidson S . GTPases of the Rho subfamily are required for Brucella abortus internalization in nonprofessional phagocytes: direct activation of Cdc42. J Biol Chem. 2001; 276(48):44435-43. DOI: 10.1074/jbc.M105606200. View

10.

Gorvel J, Moreno E . Brucella intracellular life: from invasion to intracellular replication. Vet Microbiol. 2002; 90(1-4):281-97. DOI: 10.1016/s0378-1135(02)00214-6. View

11.

Pujol M, Castillo F, Alvarez C, Rojas C, Borie C, Ferreira A . Variability in the response of canine and human dendritic cells stimulated with Brucella canis. Vet Res. 2017; 48(1):72. PMC: 5667440. DOI: 10.1186/s13567-017-0476-8. View

12.

Ferrero M, Fossati C, Baldi P . Direct and monocyte-induced innate immune response of human lung epithelial cells to Brucella abortus infection. Microbes Infect. 2010; 12(10):736-47. DOI: 10.1016/j.micinf.2010.05.002. View

13.

Yang J, Li H, Wang Z, Yu L, Liu Q, Niu X . Dihydroartemisinin inhibits multiplication of Brucella suis vaccine strain 2 in murine microglia BV2 cells via stimulation of caspase‑dependent apoptosis. Mol Med Rep. 2019; 20(5):4067-4072. PMC: 6797990. DOI: 10.3892/mmr.2019.10672. View

14.

Al Dahouk S, Kohler S, Occhialini A, Jimenez de Bagues M, Hammerl J, Eisenberg T . Brucella spp. of amphibians comprise genomically diverse motile strains competent for replication in macrophages and survival in mammalian hosts. Sci Rep. 2017; 7:44420. PMC: 5353553. DOI: 10.1038/srep44420. View

15.

Lubani M, Sharda D, Helin I . Cardiac manifestations in brucellosis. Arch Dis Child. 1986; 61(6):569-72. PMC: 1777857. DOI: 10.1136/adc.61.6.569. View

16.

Arriola Benitez P, Pesce Viglietti A, Herrmann C, Dennis V, Comerci D, Giambartolomei G . Brucella abortus Promotes a Fibrotic Phenotype in Hepatic Stellate Cells, with Concomitant Activation of the Autophagy Pathway. Infect Immun. 2017; 86(1). PMC: 5736806. DOI: 10.1128/IAI.00522-17. View

17.

Miraglia M, Rodriguez A, Barrionuevo P, Rodriguez J, Kim K, Dennis V . Traverses Brain Microvascular Endothelial Cells Using Infected Monocytes as a Trojan Horse. Front Cell Infect Microbiol. 2018; 8:200. PMC: 6011031. DOI: 10.3389/fcimb.2018.00200. View

18.

Larsen A, Nymo I, Briquemont B, Sorensen K, Godfroid J . Entrance and survival of Brucella pinnipedialis hooded seal strain in human macrophages and epithelial cells. PLoS One. 2013; 8(12):e84861. PMC: 3869908. DOI: 10.1371/journal.pone.0084861. View

19.

Ruiz-Ranwez V, Posadas D, Van der Henst C, Estein S, Arocena G, Abdian P . BtaE, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of Brucella suis. Infect Immun. 2013; 81(3):996-1007. PMC: 3584859. DOI: 10.1128/IAI.01241-12. View

20.

Macedo A, Silva A, Mol J, Costa L, Garcia L, Araujo M . The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages. PLoS One. 2015; 10(9):e0138131. PMC: 4569489. DOI: 10.1371/journal.pone.0138131. View