Meta-Analysis of Randomized Clinical Trials Evaluating Effectiveness of a Multivitamin Supplementation Against Oxidative Stress in Healthy Subjects

Overview

Journal Nutrients

Date 2022 Mar 26

PMID 35334829

Authors

Seoyoung Lee

Iksoo Huh

Seunghee Kang

Yea-Eun Nam

Youngseo Cho

Md Kamruzzaman

Jina Hong

Oran Kwon

Taesung Park

Affiliations

Soon will be listed here.

Abstract

A meta-analysis has been widely applied to draw general conclusions using a set of studies with similar purposes and designs. This study aimed to perform a meta-analysis of six randomized placebo-controlled trials, independently conducted for the relationship between a plant-based multivitamin/mineral supplementation (PMS) and oxidative stress for 6 to 8 weeks, to provide overall estimates of those effects. In detail, linear mixed model analysis was first conducted on each study to obtain individual estimates; then, two types of meta-analysis were applied to combine the individual estimates from all available studies (overall meta-analysis) and region-specific studies (subgroup meta-analysis). In the meta-analysis, we selected 19 biomarker variables that overlapped in at least two studies and found 6 variables significant in at least one meta-analysis. The overall estimates of beta coefficients were 0.17 for vitamin C, 0.80 for vitamin B, 0.46 for vitamin B, 0.81 for folate, 0.36 for -carotene, and -0.17 for oxidized LDL (ox-LDL). Subsequent association analysis revealed significant negative correlations between plasma free radical scavenging nutrients and plasma ox-LDL levels, indicating a general benefit of PMS in alleviating oxidative stress by providing exogenous oxidant scavengers.

References

Kim H . Statistical notes for clinical researchers: assessing normal distribution (2) using skewness and kurtosis. Restor Dent Endod. 2013; 38(1):52-4. PMC: 3591587. DOI: 10.5395/rde.2013.38.1.52. View

. NIH State-of-the-Science Conference Statement on Multivitamin/Mineral Supplements and Chronic Disease Prevention. NIH Consens State Sci Statements. 2007; 23(2):1-30. View

Cochran W . The comparison of percentages in matched samples. Biometrika. 1950; 37(3-4):256-66. View

Gaziano J . Antioxidants in cardiovascular disease: randomized trials. Nutr Rev. 1996; 54(6):175-7. DOI: 10.1111/j.1753-4887.1996.tb03925.x. View

Ashoori M, Saedisomeolia A . Riboflavin (vitamin B₂) and oxidative stress: a review. Br J Nutr. 2014; 111(11):1985-91. DOI: 10.1017/S0007114514000178. View

Greco T, Zangrillo A, Biondi-Zoccai G, Landoni G . Meta-analysis: pitfalls and hints. Heart Lung Vessel. 2013; 5(4):219-25. PMC: 3868184. View

Bravata D, Olkin I . Simple pooling versus combining in meta-analysis. Eval Health Prof. 2001; 24(2):218-30. DOI: 10.1177/01632780122034885. View

Burnett-Hartman A, Fitzpatrick A, Gao K, Jackson S, Schreiner P . Supplement use contributes to meeting recommended dietary intakes for calcium, magnesium, and vitamin C in four ethnicities of middle-aged and older Americans: the Multi-Ethnic Study of Atherosclerosis. J Am Diet Assoc. 2009; 109(3):422-9. PMC: 2684701. DOI: 10.1016/j.jada.2008.11.023. View

Kim J, Yang Y, Yang Y, Oh S, Hong Y, Lee E . Diet quality scores and oxidative stress in Korean adults. Eur J Clin Nutr. 2011; 65(12):1271-8. DOI: 10.1038/ejcn.2011.120. View

10.

Kang S, Lim Y, Kim Y, Jung E, Suh D, Lee C . Multivitamin and Mineral Supplementation Containing Phytonutrients Scavenges Reactive Oxygen Species in Healthy Subjects: A Randomized, Double-Blinded, Placebo-Controlled Trial. Nutrients. 2019; 11(1). PMC: 6356358. DOI: 10.3390/nu11010101. View

11.

Fletcher R, Fairfield K . Vitamins for chronic disease prevention in adults: clinical applications. JAMA. 2002; 287(23):3127-9. DOI: 10.1001/jama.287.23.3127. View

12.

Higgins J, Thompson S, Deeks J, Altman D . Measuring inconsistency in meta-analyses. BMJ. 2003; 327(7414):557-60. PMC: 192859. DOI: 10.1136/bmj.327.7414.557. View

13.

Schober P, Vetter T . Meta-Analysis in Clinical Research. Anesth Analg. 2020; 131(4):1090-1091. PMC: 7467147. DOI: 10.1213/ANE.0000000000005001. View

14.

Higgins J, Thompson S . Quantifying heterogeneity in a meta-analysis. Stat Med. 2002; 21(11):1539-58. DOI: 10.1002/sim.1186. View

15.

Nordmann A, Kasenda B, Briel M . Meta-analyses: what they can and cannot do. Swiss Med Wkly. 2012; 142:w13518. DOI: 10.4414/smw.2012.13518. View

16.

Fairfield K, Fletcher R . Vitamins for chronic disease prevention in adults: scientific review. JAMA. 2002; 287(23):3116-26. DOI: 10.1001/jama.287.23.3116. View

17.

McKay D, Perrone G, Rasmussen H, Dallal G, Hartman W, Cao G . The effects of a multivitamin/mineral supplement on micronutrient status, antioxidant capacity and cytokine production in healthy older adults consuming a fortified diet. J Am Coll Nutr. 2000; 19(5):613-21. DOI: 10.1080/07315724.2000.10718959. View

18.

Lim Y, Ahn Y, Yoo J, Park K, Kwon O . Verifying Identities of Plant-Based Multivitamins Using Phytochemical Fingerprinting in Combination with Multiple Bioassays. Plant Foods Hum Nutr. 2017; 72(3):288-293. DOI: 10.1007/s11130-017-0622-5. View

19.

Haidich A . Meta-analysis in medical research. Hippokratia. 2011; 14(Suppl 1):29-37. PMC: 3049418. View

20.

Isakov V, Bogdanova A, Bessonov V, Sentsova T, Tutelyan V, Lin Y . Effects of Multivitamin, Multimineral and Phytonutrient Supplementation on Nutrient Status and Biomarkers of Heart Health Risk in a Russian Population: A Randomized, Double Blind, Placebo Controlled Study. Nutrients. 2018; 10(2). PMC: 5852696. DOI: 10.3390/nu10020120. View