Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy

Overview

Journal Annu Rev Biochem

Publisher Annual Reviews

Specialty Biochemistry

Date 2022 Mar 23

PMID 35320683

Authors

Eugene Y D Chua

Joshua H Mendez

Micah Rapp

Serban L Ilca

Yong Zi Tan

Kashyap Maruthi

Huihui Kuang

Christina M Zimanyi

Anchi Cheng

Edward T Eng

Alex J Noble

Clinton S Potter

Bridget Carragher

Affiliations

Soon will be listed here.

Abstract

Cryo-electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomography have enabled more structures to be imaged and determined to better resolutions, at faster speeds, and with more scientists having improved access. This review highlights recent advances at each stageof the cryo-EM pipeline and provides examples of how these techniques have been used to investigate real-world problems, including antibody development against the SARS-CoV-2 spike during the recent COVID-19 pandemic.

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