Application of Droplet Digital PCR to Detection of and Infections: A Narrative Review

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2022 Mar 22

PMID 35313727

Authors

Yuxin Fan

Jingjing Chen

Meixiao Liu

Xin Xu

Yu Zhang

Peng Yue

Wenjing Cao

Zhenhua Ji

Xuan Su

Shiyuan Wen

Jing Kong

Guozhong Zhou

Bingxue Li

Yan Dong

Aihua Liu

Fukai Bao

Affiliations

Soon will be listed here.

Abstract

Tuberculosis (TB) is a chronic infectious disease caused by infection, which has seriously endangered human health for many years. With the emergence of multidrug-resistant and extensively drug-resistant , the prevention and treatment of TB has become a pressing need. Early diagnosis, drug resistance monitoring, and control of disease transmission are critical aspects in the prevention and treatment of TB. However, the currently available diagnostic technologies and drug sensitivity tests are time consuming, and thus, it is difficult to achieve the goal of early diagnosis and detection drug sensitivity, which results in limited control of disease transmission. The development of molecular testing technology has gradually achieved the vision of rapid and accurate diagnosis of TB. Droplet digital PCR (ddPCR) is an excellent nucleic acid quantification method with high sensitivity and no need for a calibration curve. Herein, we review the application of ddPCR in TB diagnosis and drug resistance detection and transmission monitoring.

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