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Can Molecular Methods Detect 1% Isoniazid Resistance in Mycobacterium Tuberculosis?

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2013 Mar 1
PMID 23447641
Citations 35
Authors
Dorte Bek Folkvardsen
Erik Svensson
Vibeke O Thomsen
Erik Michael Rasmussen
Didi Bang
Jim Werngren
Sven Hoffner
Doris Hillemann
Leen Rigouts
Affiliations
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Abstract

Patients may harbor both drug-susceptible and -resistant bacteria, representing heteroresistance. We studied mixtures of isoniazid-resistant and -susceptible Mycobacterium tuberculosis strains. Conventional drug susceptibility testing was the most sensitive method of detection, whereas the line probe assay and sequencing were not able to detect the clinically relevant 1% proportion of resistant bacteria.

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References
1.
CANETTI G, Fox W, Khomenko A, MAHLER H, Menon N, Mitchison D . Advances in techniques of testing mycobacterial drug sensitivity, and the use of sensitivity tests in tuberculosis control programmes. Bull World Health Organ. 1969; 41(1):21-43. PMC: 2427409. View
2.
Vijdea R, Stegger M, Sosnovskaja A, Andersen A, Thomsen V, Bang D . Multidrug-resistant tuberculosis: rapid detection of resistance to rifampin and high or low levels of isoniazid in clinical specimens and isolates. Eur J Clin Microbiol Infect Dis. 2008; 27(11):1079-86. DOI: 10.1007/s10096-008-0548-9. View
3.
David H . Probability distribution of drug-resistant mutants in unselected populations of Mycobacterium tuberculosis. Appl Microbiol. 1970; 20(5):810-4. PMC: 377053. DOI: 10.1128/am.20.5.810-814.1970. View
4.
Abate G, Hoffner S, Thomsen V, Miorner H . Characterization of isoniazid-resistant strains of Mycobacterium tuberculosis on the basis of phenotypic properties and mutations in katG. Eur J Clin Microbiol Infect Dis. 2001; 20(5):329-33. DOI: 10.1007/pl00011272. View
5.
Lindstrom A, Odeberg J, Albert J . Pyrosequencing for detection of lamivudine-resistant hepatitis B virus. J Clin Microbiol. 2004; 42(10):4788-95. PMC: 522336. DOI: 10.1128/JCM.42.10.4788-4795.2004. View